Expansion of Circulating T Follicular Helper Cells in Children with Acute Henoch-Schönlein Purpura

Xie, Jue; Liu, Yan; Wang, Lei; Ruan, Guangfeng; Yuan, Huiming; Fang, Hong; Wu, Jiang

doi:10.1155/2015/742535

Cited by 16 publications

(18 citation statements)

References 40 publications

(58 reference statements)

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“…PD-1, a functional marker of Tfh cells, exhibited no differences in expression between the two groups. A similar nding reported an expansion of circulating Tfh cells in children with acute HSP; a signi cant increase in CXCR5 + CD4 + cells in children with HSP and no differences in PD-1 expression between two groups were noted (12). Moreover, correlations between Tfh cells and naïve B cells, and class-switched B cell subsets, as well as memory B cells, were found in children with HSP in our study.…”

Section: Discussionsupporting

confidence: 85%

Abnormal B Cell Compartment Associated With Tfh Cells in Children With Henoch-schonlein Purpura

Zhang

Sun

Pan

et al. 2020

Preprint

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Aim IgA-producing B cells were found to be associated with children diagnosed with Henoch-Schonlein purpura (HSP). The present study aimed to determine whether children with HSP possess abnormal B cell subsets. Methods A total of 14 children diagnosed with HSP, and age- and gender-matched healthy controls were enrolled in our study. Peripheral blood mononuclear cells were isolated, and the percentage of B cells subsets and Tfh cells were determined by flow cytometry. Finally, Spearman’s correlation coefficient was used to analyze the correlation between the percentage of Tfh cells and B cell subsets. Results We found that the frequency of total B cells was significantly increased in children with HSP; however, the percentage of plasma cells was significantly lower in HSP children. A significant reduction in the count of naïve B cells and an increase in class-switched B cells were found in children with HSP compared with healthy controls. We observed that the expression of C-X-C chemokine receptor type 5 (CXCR5) on total CD4+ T cells and the percentage of CD4+CXCR5+ cells were significantly increased within HSP patients. Moreover, significant correlations between Tfh cells and various B cells subsets were observed. Conclusion Our study showed a Tfh cell-associated abnormal B cell compartment in HSP children.

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Section: Discussionsupporting

confidence: 85%

Abnormal B Cell Compartment Associated With Tfh Cells in Children With Henoch-schonlein Purpura

Zhang

Sun

Pan

et al. 2020

Preprint

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“…Moreover, serum levels of IL-21, IL-6, IgA and C3 were also significantly higher in HSP children than in healthy controls. A positive correlation was observed between the frequency of circulating ICOS + CXCR5 + CD4 + Tfh cells and the serum levels of IL-21 or IgA (74). Additionally, mRNA expression levels of IL-21, IL-6 and Bcl-6 were also significantly increased in peripheral blood from children with acute HSP compared to healthy controls (74).…”

Section: Tfh Cellsmentioning

confidence: 90%

“…A positive correlation was observed between the frequency of circulating ICOS + CXCR5 + CD4 + Tfh cells and the serum levels of IL-21 or IgA (74). Additionally, mRNA expression levels of IL-21, IL-6 and Bcl-6 were also significantly increased in peripheral blood from children with acute HSP compared to healthy controls (74). Following treatment, the numbers of CD4 + CXCR5 + , CD4 + CXCR5 + PD-1 + and CD4 + CXCR5 + ICOS + Tfh cells, as well as serum levels of IL-21 were significantly reduced (75).…”

Section: Tfh Cellsmentioning

confidence: 90%

“…Tfh cells play crucial roles in regulating immune responses. Studies have shown that the frequency of circulating CXCR5 + CD4 + Tfh cells coexpressing ICOS was significantly higher in children with acute HSP than in healthy controls, whereas the frequency of CXCR5 + CD4 + Tfh cells expressing PD-1 was not increased in these patients (74,75). Moreover, serum levels of IL-21, IL-6, IgA and C3 were also significantly higher in HSP children than in healthy controls.…”

Section: Tfh Cellsmentioning

confidence: 98%

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The role of T cells in the development of Henoch-Schonlein purpura

Wang¹

2018

Front Biosci

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“…The expansion of circulating Tfh cells has been reported in various autoimmune diseases [ 19 , 21 ], suggesting their pathogenic significance. Xie et al revealed that the frequency of circulating CD4 + CXCR5 + ICOS + Tfh cells in children with active IgAV was significantly higher than in healthy children [ 22 ]. Wang et al also reported that the upregulation of circulating Tfh cells and downregulation of circulating follicular regulatory T (Tfr) cells may contribute to the pathogenesis of IgAV in children [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Distinct phenotypic subpopulations of circulating CD4+CXCR5+ follicular helper T cells in children with active IgA vasculitis

Liu

Wang

et al. 2016

BMC Immunol

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BackgroundCirculating follicular helper T (Tfh) cells are a heterogeneous population of CD4+ helper T cells that promotes pathogenic immune responses in autoimmune diseases. In this study, we examined the status of different subpopulations of Tfh cells in peripheral circulation and their associations with various clinical characteristics of IgA vasculitis (IgAV).MethodsAccording to the phenotypic expressions of different molecules, focus was given on six subpopulations of Tfh cells: CD4+CXCR5+, CD4+CXCR5+ICOS+, CD4+CXCR5+ICOS+PD-1+, CD4+CXCR5+ICOShighPD-1high, CD4+CXCR5+ICOS−PD-1+, and CXCR5+CD45RA−IL-21+. The frequencies of these six subpopulations and the circulating level of Tfh-related cytokine interleukin 21 (IL-21) were measured from 27 patients with IgAV and 15 healthy controls (HC) by flow cytometry and flow cytometric bead array, respectively.ResultsSignificantly higher frequencies of CD4+CXCR5+, CD4+CXCR5+ICOS+, CD4+CXCR5+ICOS+PD-1+, CD4+CXCR5+ICOShighPD-1high and CXCR5+CD45RA−IL-21+ Tfh cells, as well as higher levels of plasma IL-21, were detected in IgAV patients compared to HC. The level of each Tfh subpopulation varied by the presenting symptoms of IgAV, but did not differ between patients treated or not treated with glucocorticoids. When the disease entered the remission stage following treatment, circulating levels of CD4+CXCR5+, CD4+CXCR5+ICOS+, CD4+CXCR5+ICOS+PD-1+, CD4+CXCR5+ICOShighPD-1high and CXCR5+CD45RA−IL-21+ Tfh cells, as well as plasma IL-21 levels were reduced. Among the six subpopulations of Tfh cells, both CD4+CXCR5+ICOS+ and CXCR5+CD45RA−IL-21+ significantly and positively correlated with serum IgA and plasma IL-21 levels, but only CXCR5+CD45RA−IL-21+ significantly and negatively correlated with the serum C4 level.ConclusionsTfh cells may differentially contribute to the development of IgAV or predict disease progression. These findings provide novel insights in the pathogenesis of IgAV and may benefit treatment development targeting organ-specific presenting symptoms of IgAV.

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Expansion of Circulating T Follicular Helper Cells in Children with Acute Henoch-Schönlein Purpura

Cited by 16 publications

References 40 publications

Abnormal B Cell Compartment Associated With Tfh Cells in Children With Henoch-schonlein Purpura

Abnormal B Cell Compartment Associated With Tfh Cells in Children With Henoch-schonlein Purpura

The role of T cells in the development of Henoch-Schonlein purpura

Distinct phenotypic subpopulations of circulating CD4+CXCR5+ follicular helper T cells in children with active IgA vasculitis

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