Expansion of Human Hematopoietic Cells from Umbilical Cord Blood Using Roller Bottles in CO₂and CO₂-Free Atmosphere

Abstract

In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium for cultures in CO(2)-free atmosphere were assessed. At day 5 of culture, total colony forming unit expansions of 14.44 ± 3.74, 11.20 ± 6.37, and 17.25 ± 3.65-folds were attained, respectively. The expansion reached using L-15 medium in roller bottles was around 10 times higher than that achieved in the static control cultures. To our knowledge, this is the first report of cultures in CO(2)-free atmosphere to expand cord blood human hematopoietic stem cells and it opens a new branch of possibilities for culturing and clinical applications.

3-Dimensional nano-fibre scaffold forex vivoexpansion of cord blood haematopoietic stem cells

“…They find that RBs are suitable to culture human umbilical CB MNC in all media notice above. L-15 RB had higher and longer CFC expansion (18.39-fold for 13 days) than other medium and expansion using the L-15 medium in RBs was about 10 times higher than that static control cultures [28].…”

“…In another study in 2014, Andrade-Zald ıvar et al had contrary data than 2011 and revealed that maximum total cell proliferation (26 vs. 20-folds for 10 days culture) and maximum progenitor expansion (17 vs. 16-folds for five days culture) in the static medium were higher than RB. These difference are maybe higher progenitor expansion could be found even earlier in the cultures of the enriched fractions and also, primitive cells cultured in static conditions may lose their stem features [29].…”

“…In final, Andrade-Zald ıvar et al demonstrated that RBs were suitable for the expansion of haematopoietic progenitor's cells and were simply scalable to large volumes and they were appropriate for the culture of suspended cells such as MNC. Also, RBs had the capability to achieve cultures 20 times larger than the statics and therefore 20 times more total expanded progenitors [28,29].…”

Large‐scale production of human mesenchymal stem cells for clinical applications

“…Roller bottles have been used for MSC‐based tissue engineering applications 76,77, and recently for the expansion of hMSCs and hematopoietic cells 78,79. Cohen and McNiece 79 demonstrated enhanced expansion of hMSCs in roller bottles with no significant changes in morphology, but reduced expression of interleukin‐1 and interleukin‐6, compared with those grown in T‐flasks.…”

Stem cell bioengineering strategies to widen the therapeutic applications of haematopoietic stem/progenitor cells from umbilical cord blood

“…Cellmate TM (Bernard et al ., ; Kunitake et al ., ). Very recently, Andrade‐Zaldivar et al ., ) reported a 17‐fold increase in CFU after 8 days of expansion of UCB MNCs using roller bottles (Andrade‐Zaldivar et al ., ).…”