Expansion of the host range of coliphage P1 and gene transfer from enteric bacteria to other gram-negative bacteria

Murooka, Yoshikatsu; Harada, Tokuya

doi:10.1128/aem.38.4.754-757.1979

Cited by 41 publications

(17 citation statements)

References 20 publications

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“…It is expected that phages can inject DNA into a broader range of hosts than those in which phage replication is observed macroscopically as plaques, because producing phage progeny requires many steps beyond DNA injection. Plaques are observed only if the phage has resisted bacterial defenses (restriction, CRISPR, superinfection exclusion); has expressed, processed, and assembled its components; and has lysed the host cell (65,66).…”

Section: Phages As Dna Delivery Shuttlesmentioning

confidence: 99%

Phage-Based Applications in Synthetic Biology

2018

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Bacteriophage research has been instrumental to advancing many fields of biology, such as genetics, molecular biology, and synthetic biology. Many phage-derived technologies have been adapted for building gene circuits to program biological systems. Phages also exhibit significant medical potential as antibacterial agents and bacterial diagnostics due to their extreme specificity for their host, and our growing ability to engineer them further enhances this potential. Phages have also been used as scaffolds for genetically programmable biomaterials that have highly tunable properties. Furthermore, phages are central to powerful directed evolution platforms, which are being leveraged to enhance existing biological functions and even produce new ones. In this review, we discuss recent examples of how phage research is influencing these next-generation biotechnologies.

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Section: Phages As Dna Delivery Shuttlesmentioning

confidence: 99%

Phage-Based Applications in Synthetic Biology

2018

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“…P1 only infects E. coli, which normally restricts its use to this species. However, some variants of P1 have been developed that have a broader host range and can infect other species within the family Enterobacteriaceae, and even some other γ-proteobacterial species, albeit at reduced efficiency 36,37 . Even the transduction of cloned DNA from E. coli to Myxococcus xanthus, a δproteobacterium, has been reported 38 .…”

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Producing Gene Deletions in <em>Escherichia coli</em> by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Saragliadis

Trunk

Leo

2018

JoVE

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A first approach to study the function of an unknown gene in bacteria is to create a knock-out of this gene. Here, we describe a robust and fast protocol for transferring gene deletion mutations from one Escherichia coli strain to another by using generalized transduction with the bacteriophage P1. This method requires that the mutation be selectable (e.g., based on gene disruptions using antibiotic cassette insertions). Such antibiotic cassettes can be mobilized from a donor strain and introduced into a recipient strain of interest to quickly and easily generate a gene deletion mutant. The antibiotic cassette can be designed to include flippase recognition sites that allow the excision of the cassette by a site-specific recombinase to produce a clean knock-out with only a ~100-base-pair-long scar sequence in the genome. We demonstrate the protocol by knocking out the tamA gene encoding an assembly factor involved in autotransporter biogenesis and test the effect of this knock-out on the biogenesis and function of two trimeric autotransporter adhesins. Though gene deletion by P1 transduction has its limitations, the ease and speed of its implementation make it an attractive alternative to other methods of gene deletion.

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“…Previously, we extended the host range of coliphage P1 (25) and created intergeneric hybrid strains of enteric bacteria (26). However, the arylsulfatase gene, atsK, from Klebsiella aer-ogenes could not be transferred to E. coli, because the DNA homology of the ats genes of these two bacteria is low.…”

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confidence: 99%

Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.

Murooka¹,

Takizawa²,

Harada³

1981

Journal of Bacteriology

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The host range of coliphage Mu was greatly expanded to various genera of gram-negative bacteria by using the hybrid plasmid RP4::Mu cts, which is temperature sensitive and which confers resistance to ampicillin, kanamycin, and tetracycline. These drug resistance genes were transferred from Escherichia coli to members of the genera Klebsiella, Enterobacter, Citrobacter, Salmonella, Proteus, Erwinia, Serratia, Alcaligenes, Agrobacterium, Rhizobium, Pseudomonas, Acetobacter, and Bacillus. Mu phage was produced by thermal induction from the lysogens of all these drug-resistant bacteria except Bacillus. Mu phage and RP4 or the RP4: :Mu plasmid were used to create intergeneric recombinant strains by transfer of some genes, including the arylsulfatase gene, between Klebsiella aerogenes and E. coli. Thus, genetic analysis and intergeneric gene transfer are possible in these RP4: :Mu-sensitive bacteria.

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Expansion of the host range of coliphage P1 and gene transfer from enteric bacteria to other gram-negative bacteria

Cited by 41 publications

References 20 publications

Phage-Based Applications in Synthetic Biology

Phage-Based Applications in Synthetic Biology

Producing Gene Deletions in <em>Escherichia coli</em> by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.

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