Expeditious Quantification of Lignocellulolytic Enzymes from Indigenous Wood Rot and Litter Degrading Fungi from Tropical Dry Evergreen Forests of Tamil Nadu

Abstract

In this study thirty wood rotting and litter degrading basidiomycetes were screened for the production of lignocellulolytic enzymes such as, laccase, peroxidase, and cellulase using rapid micro quantification assay. Out of the 30 indigenous isolates Trametes gibbosa was identified to be a potential lignocellulolytic enzyme producer, producing a maximum amount of cellulase (299.66 ± 1.59 IU/L) and laccase (257.94 ± 1.79 U/L). Moreover, it is the second leading producer of peroxidase enzyme (170.19 ± 1.98 U/L). Tricholomopsis sp. a wood rot basidiomycete was found to be the leading lignin decomposer with maximum peroxidase activity (287.84 ± 2 U/L) and second maximum laccase activity (250.19 ± 1.83 U/L). However, its cellulolytic potential was found to be moderate (100.04 ± 1.13 U/L). A higher level of lignocellulolytic enzymes was recorded in wood rotting basidiomycetes, whereas very low levels of lignolytic enzymes were found in litter inhabiting basidiomycetes. However, their cellulolytic potential was found to be moderate.

Fungi.guru: Comparative genomic and transcriptomic resource for the fungi kingdom

“…Their main source of energy comes from digesting decomposing plant material such as cellulose and lignin [51] . Basidiomycetous fungi, for example, Coprinopsis cinerea (Cci) [52] , are one of the most potent degraders of cellulose as they often grow in environments that are cellulose-abundant, such as dead wood and plants [53] , [54] . Plant cell walls are multi-layered and made up of different materials which include cellulose, hemicelluloses, and lignins [55] , leading to the need for fungi to produce various types of hydrolytic enzymes (endoglucanase, cellobiohydrolase, and beta-glucosidase) to break down these biomolecules into substances that can be used as sources of energy for cellular activity [56] .…”

Fungi.guru: comparative genomic and transcriptomic database for the Fungi kingdom

“…Their main source of energy comes from digesting decomposing plant material such as cellulose and lignin (43). Basidiomycetous fungi, for example, Coprinopsis cinerea (Cci) (44), are one of the most potent degraders of cellulose as they often grow in environments that are cellulose-abundant, such as dead wood and plants (45,46). Plant cell walls are multi-layered and made up of different materials which include cellulose, hemicelluloses, and lignins (47), leading to the need for fungi to produce various types of hydrolytic enzymes (endoglucanase, cellobiohydrolase, and beta-glucosidase) to break down these biomolecules into substances that can be used as sources of energy for cellular activity (48).…”

Transcriptomics and co-expression network analysis revealing candidate genes for the laccase activity of Trametes gibbosa

“…In wild, T. gibbosa secretes manganese peroxidase and laccase to participate in wood decay and litter degradation [ 27 , 28 ]. In this study, the RNA-seq data analysis of T. gibbosa (CB-1) with wood chips ( Populus simonii × P.nigra ) was performed.…”