Experience-dependent development of visual sensitivity in larval zebrafish

Xie, Jiaheng; Jusuf, Patricia Regina; Bui, Bang V.; Goodbourn, Patrick T.

doi:10.1038/s41598-019-54958-6

Cited by 24 publications

(15 citation statements)

References 58 publications

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“…Experiments in humans and mammalian models are, however, constrained by the limitations described in the introduction. CPs have therefore been identified and interrogated in a number of models more conducive to experimentation: Danio rario ( Riley et al, 1997 ; Moorman et al, 2002 ; Avitan et al, 2017 ; Xie et al, 2019 ); H. americanus ( Govind and Pearce, 1989 ); C. elegans ( Swanson and Riddle, 1981 ), and Drosophila ( Fushiki et al, 2013 ; Marley and Baines, 2011 ; Giachello and Baines, 2015 ). The identification of CPs in the fruit fly means that the A27h-related larval locomotor circuit provides an exciting opportunity to provide network, cellular and mechanism-level resolution insight into the role of CPs in neurodevelopment.…”

Section: Using the A27h-related Larval Locomotor Circuit To Model Critical Periods Of Neural Developmentmentioning

confidence: 99%

The Drosophila Larval Locomotor Circuit Provides a Model to Understand Neural Circuit Development and Function

Hunter

Coulson

Zarin

et al. 2021

Front. Neural Circuits

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It is difficult to answer important questions in neuroscience, such as: “how do neural circuits generate behaviour?,” because research is limited by the complexity and inaccessibility of the mammalian nervous system. Invertebrate model organisms offer simpler networks that are easier to manipulate. As a result, much of what we know about the development of neural circuits is derived from work in crustaceans, nematode worms and arguably most of all, the fruit fly, Drosophila melanogaster. This review aims to demonstrate the utility of the Drosophila larval locomotor network as a model circuit, to those who do not usually use the fly in their work. This utility is explored first by discussion of the relatively complete connectome associated with one identified interneuron of the locomotor circuit, A27h, and relating it to similar circuits in mammals. Next, it is developed by examining its application to study two important areas of neuroscience research: critical periods of development and interindividual variability in neural circuits. In summary, this article highlights the potential to use the larval locomotor network as a “generic” model circuit, to provide insight into mammalian circuit development and function.

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Section: Using the A27h-related Larval Locomotor Circuit To Model Critical Periods Of Neural Developmentmentioning

confidence: 99%

The Drosophila Larval Locomotor Circuit Provides a Model to Understand Neural Circuit Development and Function

Hunter

Coulson

Zarin

et al. 2021

Front. Neural Circuits

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“…114 Using OMR, it was recently demonstrated for zebrafish, as has been shown for mouse, 123 that dynamic visual experience is required for the proper stratification of retinal layers in early eye maturation. 124 Studies have shown that the OMR displayed by larval zebrafish is lost around 16 dpf and that adult zebrafish rely mainly on mechanosensory stimuli via the lateral line for sensing movement, and on positive rheotaxis (defined as the tendency to turn to face into an oncoming current) for maintaining position in flowing water. 125 Larval zebrafish also display positive rheotaxis.…”

Section: Functional Vision Testingmentioning

confidence: 99%

Zebrafish as an Animal Model for Ocular Toxicity Testing: A Review of Ocular Anatomy and Functional Assays

2020

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Xenobiotics make their way into organisms from diverse sources including diet, medication, and pollution. Our understanding of ocular toxicities from xenobiotics in humans, livestock, and wildlife is growing thanks to laboratory animal models. Anatomy and physiology are conserved among vertebrate eyes, and studies with common mammalian preclinical species (rodent, dog) can predict human ocular toxicity. However, since the eye is susceptible to toxicities that may not involve a histological correlate, and these species rely heavily on smell and hearing to navigate their world, discovering visual deficits can be challenging with traditional animal models. Alternative models capable of identifying functional impacts on vision and requiring minimal amounts of chemical are valuable assets to toxicology. Human and zebrafish eyes are anatomically and functionally similar, and it has been reported that several common human ocular toxicants cause comparable toxicity in zebrafish. Vision develops rapidly in zebrafish; the tiny larvae rely on visual cues as early as 4 days, and behavioral responses to those cues can be monitored in high-throughput fashion. This article describes the comparative anatomy of the zebrafish eye, the notable differences from the mammalian eye, and presents practical applications of this underutilized model for assessment of ocular toxicity.

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“…The OMR apparatus was adapted from one previously described (Supplementary Figure S2) [21,22]. Figure S2a).…”

Section: Optomotor Response (Omr) (A) Apparatus and Proceduresmentioning

confidence: 99%

Altered visual function in a larval zebrafish knockout of neurodevelopmental risk genepdzk1

Xie

Jusuf

Bui

et al. 2020

Preprint

Self Cite

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The human PDZK1 gene is located in a genomic susceptibility region for neurodevelopmental disorders. A genome-wide association study (GWAS) identified links between PDZK1 polymorphisms and altered visual contrast sensitivity, an endophenotype for schizophrenia and autism spectrum disorder. The PDZK1 protein is implicated in neurological functioning, interacting with synaptic molecules including post-synaptic density 95 (PSD-95), N-methyl-D-aspartate receptors (NMDAR), corticotropin-releasing factor receptor 1 (CRFR1) and serotonin 2A receptors. To elucidate the role of PDZK1, we generated pdzk1-knockout (pdzk1-KO) zebrafish using CRISPR/Cas-9 genome editing. Visual function of 7-day-old fish was assessed at behavioural and functional levels using the optomotor response (OMR) and scotopic electroretinogram (ERG). We also quantified retinal morphology and densities of PSD-95, NMDAR1, CRFR1 and serotonin in the synaptic inner plexiform layer at 7 days, 4 weeks and 8 weeks of age. Relative to wild-type, pdzk1-KO larvae showed spatial-frequency tuning functions with increased amplitude (likely due to abnormal gain control) and reduced ERG b-waves (suggestive of inner retinal dysfunction). However, these functional differences were not associated with gross synaptic or morphological retinal phenotypes. The findings corroborate a role for pdzk1 in visual function, and our model system provides a platform for investigating other genes associated with abnormal visual behaviour.

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Experience-dependent development of visual sensitivity in larval zebrafish

Cited by 24 publications

References 58 publications

The Drosophila Larval Locomotor Circuit Provides a Model to Understand Neural Circuit Development and Function

The Drosophila Larval Locomotor Circuit Provides a Model to Understand Neural Circuit Development and Function

Zebrafish as an Animal Model for Ocular Toxicity Testing: A Review of Ocular Anatomy and Functional Assays

Altered visual function in a larval zebrafish knockout of neurodevelopmental risk genepdzk1

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