The COVID-19 pandemic massively changed the context and feasibility of developmental research. This new reality as well as considerations, e.g., about sample diversity and naturalistic settings for developmental research, indicate the need for solutions for online studies. In this article, we present e-Babylab, an open-source browser-based tool for unmoderated online studies specifically targeted at studying young children and babies. e-Babylab offers an intuitive graphical user interface for study creation and management of studies, users, participant data, and stimulus material with no programming skills required. Various kinds of audiovisual media can be presented as stimuli and possible measures include webcam recordings, audio recordings, key presses, mouse-click/touch coordinates, and reaction times. Information pages, consent forms, and participant forms are all customizable. e-Babylab was used with a variety of measures and paradigms in 14 studies with children aged 12 months to 8 years (n = 1516). We briefly summarize some results of these studies to demonstrate data quality and that participants’ engagement and the overall results are comparable in lab and online settings. We then present in more detail one replication of an established preferential-looking paradigm with gaze directions manually coded from the webcam captures. Finally, we discuss useful tips for using e-Babylab and present plans for upgrades.