The use of modern information and communication technology plays a significant role in healthcare services improvement. In the recent years, various mobile application systems have been deployed in the health sectors of different developing countries to facilitate remote data collection and transmission so as to improve its quality and availability. Consequently, understanding the factors contributing to mobile technology acceptance is imperative. The purpose of this study was to adopt a modified UTAUT theoretical model to understand the factors influence acceptance and use of mobile health applications by health workers at health facilities in Tanzania. Questionnaires were used to collect data from health facilities workers. Out of 150 health facilities workers, only 108 return, a 72% return rate whose data was statistically analyzed using SPSS tool. The findings show that effort expectancy and facilitating conditions significantly influence the users located in the urban area on behavioral intention to use mobile health applications. Furthermore, the study shows that the constructs such as social influence, training adequacy, and voluntariness of use do not have a significant influence on the use of mobile health applications.