Experimental aerogenic Burkholderia mallei (glanders) infection in the BALB/c mouse

Lever, Mark S.; Nelson, Michelle; Ireland, Philip I.; Stagg, Anthony J.; Beedham, Richard J.; Hall, G.A.; Knight, Georgina; Titball, Richard W.

doi:10.1099/jmm.0.05180-0

Cited by 40 publications

(52 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Less consistently affected are brain, kidney, skin, and skeletal muscle (32). Additionally, the histopathologic findings in the RMs and AGMs support previous findings in NHPs and murine models, which have found that the lungs, lymphatic system, and spleen are commonly affected (19,23,27,29). Overall, the histopathology findings in the RMs and AGMs are in good agreement with those of both human and other NHP models (27,29,32).…”

Section: Discussionsupporting

confidence: 80%

“…The most widely used melioidosis model system is the mouse. The murine model has been used to study many aspects of the host-pathogen interaction, which includes host immune system response, cytokine expression, tissue tropism, disease pathology, and disease progression (5,13,16,19,21,23,30,35,37). There are few reports of NHP models being used to study melioidosis, but one such study has suggested that rhesus macaques (RMs) challenged by subcutaneous injection with B. pseudomallei were relatively resistant (28).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Natural History of Inhalation Melioidosis in Rhesus Macaques (Macaca mulatta) and African Green Monkeys (Chlorocebus aethiops)

et al. 2012

View full text Add to dashboard Cite

ABSTRACTBurkholderia pseudomallei, the causative agent of melioidosis, is recognized as a serious health threat due to its involvement in septic and pulmonary infections in areas of endemicity and is recognized by the Centers for Disease Control and Prevention as a category B biothreat agent. An animal model is desirable to evaluate the pathogenesis of melioidosis and medical countermeasures. A model system that represents human melioidosis infections is essential in this process. A group of 10 rhesus macaques (RMs) and 10 African green monkeys (AGMs) was exposed to aerosolizedB. pseudomallei1026b. The first clinical signs were fever developing 24 to 40 h postexposure followed by leukocytosis resulting from a high percentage of neutrophils. Dyspnea manifested 2 to 4 days postexposure. In the AGMs, an increase in interleukin 1β (IL-1β), IL-6, IL-8, gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α) was observed. In the RMs, IL-1β, IL-6, and TNF-α increased. All the RMs and AGMs had various degrees of bronchopneumonia, with inflammation consisting of numerous neutrophils and a moderate number of macrophages. Both the RMs and the AGMs appear to develop a melioidosis infection that closely resembles that seen in acute human melioidosis. However, for an evaluation of medical countermeasures, AGMs appear to be a more appropriate model.

show abstract

Section: Discussionsupporting

confidence: 80%

mentioning

confidence: 99%

Natural History of Inhalation Melioidosis in Rhesus Macaques (Macaca mulatta) and African Green Monkeys (Chlorocebus aethiops)

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The aerosol LD 50 of B. mallei ATCC 23344 in mice is ∼10 3 bacteria [30,31] and the intraperitoneal (ip) LD 50 is ∼10 6 bacteria [32]. Given the increased virulence of aerosolized B. mallei, the goal of this study was to protect mice against an aerosol glanders challenge.…”

Section: Discussionmentioning

confidence: 99%

“…BALB/c mice are susceptible to infection with B. mallei ATCC 23344 given by aerosol and the LD 50 is approximately 10 3 bacteria [30,31]. Animals vaccinated with ILV1 or DD3008 (see above) were exposed to a high-dose aerosol challenge with ATCC 23344 3 weeks postboost.…”

Section: Vaccination With Ilv1 But Not Dd3008 Protected Against a Hmentioning

confidence: 99%

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Ulrich

Amemiya

Waag

et al. 2005

Vaccine

View full text Add to dashboard Cite

“…Mice have proven to be valuable models for Burkholderia vaccine development (Fritz et al, 2000;Jeddeloh et al, 2003;Lever et al, 2003;Ulrich et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Pathological findings and diagnostic implications of a rhesus macaque (Macacca mulatta) model of aerosol exposure to Burkholderia mallei (glanders)

Yingst

Facemire

Chuvala

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

Burkholderia mallei is a Gram-negative bacillus that causes a pneumonic disease known as glanders in equids and humans, and a lymphatic infection known as farcy, primarily in equids. With the potential to infect humans by the respiratory route, aerosol exposure can result in severe, occasionally fatal, pneumonia. Today, glanders infections in humans are rare, likely due to less frequent contact with infected equids than in the past. Acutely ill humans often have non-specific clinical signs and in order to diagnose cases, especially in scenarios of multiple cases in an unexpected setting, rapid diagnostics for B. mallei may be critical. The pathogenesis of acute glanders in the rhesus macaque (Macaca mulatta) was studied as an initial effort to improve diagnostic methods. In the study described here, the diagnostic techniques of PCR, culture and histopathology were compared. The results indicated that PCR may provide rapid, non-invasive diagnosis of glanders in some cases. As expected, PCR results were positive in lung tissue in 11/12 acutely infected rhesus macaques, but more importantly in terms of diagnostic algorithm development, PCR results were frequently positive in non-invasive samples such as broncho-alveolar lavage or nasal swabs (7/12) and occasionally in blood (3/12). However, conventional bacterial culture failed to recover bacteria in many of these samples. The study showed that the clinical presentation of aerosol-exposed rhesus macaques is similar to descriptions of human glanders and that PCR has potential for rapid diagnosis of outbreaks, if not individual cases.

show abstract

Experimental aerogenic Burkholderia mallei (glanders) infection in the BALB/c mouse

Cited by 40 publications

References 13 publications

Natural History of Inhalation Melioidosis in Rhesus Macaques (Macaca mulatta) and African Green Monkeys (Chlorocebus aethiops)

Natural History of Inhalation Melioidosis in Rhesus Macaques (Macaca mulatta) and African Green Monkeys (Chlorocebus aethiops)

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Pathological findings and diagnostic implications of a rhesus macaque (Macacca mulatta) model of aerosol exposure to Burkholderia mallei (glanders)

Contact Info

Product

Resources

About