Ultra-high-performance concrete (UHPC) has been regarded as promising alternative to provide reliable connections between difference segments (e.g., columns and pier footing/cap) during accelerated bridge construction (ABC) procedures. This paper proposes an innovative layered-UHPC connection for the prefabricated segmental (PFS) pier, whose seismic performance was validated through quasi-static experiment. The corresponding design procedure for PFS pier with this type of connections is presented based on the test results. The layered-UHPC connection ensures the emulative performance of prefabricated bridge as cast-in-place (CIP) ones, as well as provides greater economic e ciency than traditional UHPC connections. Based on experimental results, key issues concerning this connection, including the tensile behavior of UHPC, height of connection region, thickness of UHPC layer and steel bars in grouting bed, are presented and discussed. Then a seismic design procedure is proposed utilizing the capacity protection philosophy widely adopted in design speci cations. The layered-UHPC connection is expected as capacity-protected component without damage, since it provides anchorage for steels extended from columns and pier cap/footing. While the pre-fabricated region is designed as ductile component undergoing nonlinearity during strong earthquakes. Following the detailed elaborations about the design philosophy, requirement and implementation steps, this procedure is further presented through illustration examples using PFS piers with various heights. The results show that PFS piers designed according to this procedure could meet the requirement under both frequent and rare earthquakes. Note that the PFS piers with this layered-UHPC connections could be designed similar to and emulative as CIP ones, which is believed friendly to designers in engineering practice.