Experimental and Biological Insights from Proteomic Analyses of Extracellular Vesicle Cargos in Normalcy and Disease

Charest, Al

doi:10.1002/adbi.202000069

Cited by 11 publications

(11 citation statements)

References 205 publications

(357 reference statements)

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“…For instance, another important application of EVs proteomic profiling is the identification of disease-associated diagnostic and prognostic markers, as EVs proteome is both cell and disease-type dependent. Indeed, EVs are also capable of shaping the microenvironment as active players also in pathological events, such as metastatic promotion and tumor growth promotion [ 66 , 67 ]. The active involvement of EVs in cancer homeostasis and the fact that their composition reflects the contents of cell of origin make them optimal candidates for biomarker profiling studies.…”

Section: Extracellular Vesicle As Resourceful Biological Systemsmentioning

confidence: 99%

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

et al. 2022

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In the era of multi-omic sciences, dogma on singular cause-effect in physio-pathological processes is overcome and system biology approaches have been providing new perspectives to see through. In this context, extracellular vesicles (EVs) are offering a new level of complexity, given their role in cellular communication and their activity as mediators of specific signals to target cells or tissues. Indeed, their heterogeneity in terms of content, function, origin and potentiality contribute to the cross-interaction of almost every molecular process occurring in a complex system. Such features make EVs proper biological systems being, therefore, optimal targets of omic sciences. Currently, most studies focus on dissecting EVs content in order to either characterize it or to explore its role in various pathogenic processes at transcriptomic, proteomic, metabolomic, lipidomic and genomic levels. Despite valuable results being provided by individual omic studies, the categorization of EVs biological data might represent a limit to be overcome. For this reason, a multi-omic integrative approach might contribute to explore EVs function, their tissue-specific origin and their potentiality. This review summarizes the state-of-the-art of EVs omic studies, addressing recent research on the integration of EVs multi-level biological data and challenging developments in EVs origin.

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Section: Extracellular Vesicle As Resourceful Biological Systemsmentioning

confidence: 99%

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

et al. 2022

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“…Several groups reported multi-omic analysis of MPs, thereby analyzing the protein and lipid content of MPs ( 77 – 80 ). Kowal and colleagues showed that proteomic analysis of MPs using mass spectrometry led to the identification of proteins defining certain subtypes of vesicles ( 77 ).…”

Section: Experimental Techniques To Isolate Quantify and Characterize...mentioning

confidence: 99%

Microparticles in Autoimmunity: Cause or Consequence of Disease?

et al. 2022

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Microparticles (MPs) are small (100 nm – 1 um) extracellular vesicles derived from the plasma membrane of dying or activated cells. MPs are important mediators of intercellular communication, transporting proteins, nucleic acids and lipids from the parent cell to other cells. MPs resemble the state of their parent cells and are easily accessible when released into the blood or urine. MPs also play a role in the pathogenesis of different diseases and are considered as potential biomarkers. MP isolation and characterization is technically challenging and results in different studies are contradictory. Therefore, uniform guidelines to isolate and characterize MPs should be developed. Our understanding of MP biology and how MPs play a role in different pathological mechanisms has greatly advanced in recent years. MPs, especially if derived from apoptotic cells, possess strong immunogenic properties due to the presence of modified proteins and nucleic acids. MPs are often found in patients with autoimmune diseases where MPs for example play a role in the break of immunological tolerance and/or induction of inflammatory conditions. In this review, we describe the main techniques to isolate and characterize MPs, define the characteristics of MPs generated during cell death, illustrate different mechanism of intercellular communication via MPs and summarize the role of MPs in pathological mechanisms with a particular focus on autoimmune diseases.

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“…Another important application of EVs proteomic profiling is the identification of disease-associated diagnostic and prognostic markers, as EVs proteome is both cell and disease-type dependent. Indeed, EVs are also capable of shaping the microenvironment as active players also in pathological events, such as metastatic promotion and tumor growth promotion [57,58]. The active involvement of EVs in cancer homeostasis and the fact that their composition reflects the contents of cell of origin make them optimal candidates for biomarker profiling studies.…”

Section: Proteomicsmentioning

confidence: 99%

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

Shaba¹,

Vantaggiato²,

Governini³

et al. 2022

Preprint

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In the era of multi-omic sciences, dogma on singular cause-effect in physio-pathological processes is overcome and system biology approaches have been providing new perspectives to see through. In this context, extracellular vesicles (EVs) are offering a new level of complexity, given their role in cellular communication and their activity as mediators of specific signals to target cells or tissues. Indeed, their heterogeneity in terms of content, function, origin and potentiality contribute to the cross-interaction of almost every molecular process occurring in a complex system. Such features make EVs proper biological systems being, therefore, optimal targets of omic sciences. Currently, most studies focus on dissecting EVs content in order to either characterize it or to explore its role in various pathogenic processes at transcriptomic, proteomic, metabolomic, lipidomic and genomic levels. Despite valuable results are being provided by individual omic studies, the categorization of EVs biological data might represent a limit to be overcome. For this reason, a multi-omic integrative approach might contribute to explore EVs function, their tissue-specific origin and their potentiality. This review summarizes the state-of-the-art of EVs omic studies, addressing recent research on the integration of EVs multi-level biological data and challenging developments in EVs origin.

show abstract

Experimental and Biological Insights from Proteomic Analyses of Extracellular Vesicle Cargos in Normalcy and Disease

Abstract: 2000069 (5 of 16) reports [130,131] of noticeable reduction in high-abundance proteins from biofluids using ultrafiltration, making this approach very attractive for downstream MS applications.

Cited by 11 publications

References 205 publications

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

Microparticles in Autoimmunity: Cause or Consequence of Disease?

Multi-Omics Integrative Approach of Extracellular Vesicles: A Future Challenging Milestone

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