Experimental and computational approach to the rational monitoring of hydrogen-bonding interaction of 2-Imidazolidinethione with DNA and guanine

Ahmadi, Farhad; Alizadeh, Ali; Bakhshandeh‐Saraskanrood, Fatemeh; Jafari, Batool; Khodadadian, Mehdi

doi:10.1016/j.fct.2009.09.010

Cited by 50 publications

(21 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the presence of ct-DNA, the UV spectrum shows a decrease in the peak intensity at 331 nm. No redshift was observed in the UV spectra, which indicates that the binding mode is not intercalation and the hypochromism indicates that the binding mode of 5-ASA to DNA might be groovebinding [29]. The intrinsic binding constant, K b , for 5-ASA was calculated from Wolfe-Shimmer …”

Section: Absorption Studies Uv-vis Absorption Measurement Is a Simplmentioning

confidence: 99%

Synthesis, characterization and comparative DNA interaction studies of new copper(II) and nickel(II) complexes containing mesalamine drug using molecular modeling and multispectroscopic methods

Shahabadi

Fili

Shahlaei

2015

Journal of Coordination Chemistry

View full text Add to dashboard Cite

Complexes of copper(II) and nickel(II) containing the drug mesalamine (5-ASA) have been synthesized and characterized by FT-IR, mass and UV-vis spectra, elemental analysis and theoretical methods. The binding interactions between mesalamine and its Cu(II) and Ni(II) complexes with calf thymus DNA (ct-DNA) were investigated using absorption, fluorescence emission and circular dichroism spectroscopies, and viscosity measurements. Absorption spectra of 5-ASA, Cu(II) and Ni(II) complexes showed hypochromism. The calculated binding constants (K b ) obtained from UV-vis absorption studies were 1.27×10 3 , 1.6×10 3 and 1.2×10 4 M -1 for 5-ASA, Cu(II) and Ni(II) complexes, respectively. The compounds induced detectable changes in the CD spectra of ct-DNA (B→A structural transition, B→C structural transition and stabilization of the right-handed B form, for mesalamine, Cu(II) and Ni(II) complexes, respectively). The competitive binding experiments with Hoechst 33258 indicated that 5-ASA and copper complex could interact as groove binders. Furthermore, Ni complex had no effect on the fluorescence intensity and peak position of MB-DNA system. Finally, the results obtained from experimental and molecular modeling showed that complexes bind to DNA via minor groove binding.

show abstract

Section: Absorption Studies Uv-vis Absorption Measurement Is a Simplmentioning

confidence: 99%

Synthesis, characterization and comparative DNA interaction studies of new copper(II) and nickel(II) complexes containing mesalamine drug using molecular modeling and multispectroscopic methods

Shahabadi

Fili

Shahlaei

2015

Journal of Coordination Chemistry

View full text Add to dashboard Cite

show abstract

“…The bonding constant of DNA with Fenitrothion (K) was obtained from the change of DNA absorption at 258 nm according to our pervious work [21]. For weak binding affinities, the data were treated using linear reciprocal plots based on the Equation 16 [49]: …”

Section: Uv-vis Spectroscopymentioning

confidence: 99%

“…This information is valuable for evaluation of carcinogenic and mutagenic effects of pesticides or other molecules on DNA [22][23][24][25][26]. Recently in our laboratory we developed the in-vitro spectroscopic and voltammetric techniques to determine the interaction mode of pesticides with DNA [19][20][21]27]. The results perfectly showed that the pesticides generally caused a significant degree of structural perturbation to the geometry of DNA molecules.…”

Section: Introductionmentioning

confidence: 99%

Voltammetry and Spectroscopy Study of In Vitro Interaction of Fenitrothion with DNA

Ahmadi

Jafari

2010

Electroanalysis

View full text Add to dashboard Cite

The interaction of Fenitrothion with DNA has been studied using the voltammetric and spectroscopic methods. An irreversible reduction was concluded from the CV data of Fenitrothion. The values of (an) , respectively. Also the voltammetric, spectroscopic, thermodynamic and activation energy of DNA-Fenitrothion complex revealed that the mode of interaction might be partial-intercalative and complex formation is entropy favored.

show abstract

“…The ratio must be more than 1.8, which indicate that DNA is fully free of protein. DNA concentration (Molar per nucleotide) of the stock solution is determined by UV spectrophotometery using the molar absorption coefficient 7000M −1 cm −1 at 258 nm (Ahmadi, & et al, 2010).…”

Section: The Preparation Of Dna Solution and Measuring Concentrationmentioning

confidence: 99%

In-Vivo and In-Vitro Methods for Evaluation of Pesticides on DNA Structure

Ahmadi¹

2011

Pesticides - The Impacts of Pesticides Exposure

View full text Add to dashboard Cite

Experimental and computational approach to the rational monitoring of hydrogen-bonding interaction of 2-Imidazolidinethione with DNA and guanine

Cited by 50 publications

References 70 publications

Synthesis, characterization and comparative DNA interaction studies of new copper(II) and nickel(II) complexes containing mesalamine drug using molecular modeling and multispectroscopic methods

Synthesis, characterization and comparative DNA interaction studies of new copper(II) and nickel(II) complexes containing mesalamine drug using molecular modeling and multispectroscopic methods

Voltammetry and Spectroscopy Study of In Vitro Interaction of Fenitrothion with DNA

In-Vivo and In-Vitro Methods for Evaluation of Pesticides on DNA Structure

Contact Info

Product

Resources

About