Experimental and field evaluation of a live vaccine against avian pneumovirus

Patnayak, Devi P.; Sheikh, Anwar M.; Gulati, Baldev R.; Goyal, Sagar M.

doi:10.1080/0307945022041651

Cited by 36 publications

(27 citation statements)

References 21 publications

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“…Infection with goose15a/01 elicited a weak humoral response, similar to findings observed with other metapneumoviruses (19,30). Six of 18 (33%) of the day-old turkeys infected with goose 15a/01 had detectable aMPV-specific antibodies 14 days p.i.…”

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confidence: 55%

“…For example, 25 passages of subtype A virus in monkey Vero cells produced an attenuated virus that did not induce clinical signs but was protective against virulent virus challenge, whereas 98 passages of the virus in turkey tracheal organ cultures did not attenuate the virus (44,45). In the United States, a Vero cell-attenuated aMPV/C, passaged 63 times and producing no clinical signs, was recently licensed by the U.S. Department of Agriculture for vaccination of commercial turkeys (30). However, similar to the Vero cell-passaged subtype A vaccine used in Europe, the U.S. vaccine does not induce a strong humoral response, is only partially protective against an experimental virulent challenge, and, under field conditions, appears to reduce the severity of clinical signs but not the number of infected birds (19,25,30).…”

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confidence: 99%

“…In the United States, a Vero cell-attenuated aMPV/C, passaged 63 times and producing no clinical signs, was recently licensed by the U.S. Department of Agriculture for vaccination of commercial turkeys (30). However, similar to the Vero cell-passaged subtype A vaccine used in Europe, the U.S. vaccine does not induce a strong humoral response, is only partially protective against an experimental virulent challenge, and, under field conditions, appears to reduce the severity of clinical signs but not the number of infected birds (19,25,30). Studies have shown that the two major surface proteins of paramyxoviruses, fusion (F) and G, are the most important antigens for the induction of protective immune response (15,16,29).…”

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confidence: 99%

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A Wild Goose Metapneumovirus Containing a Large Attachment Glycoprotein Is Avirulent but Immunoprotective in Domestic Turkeys

Bennett

LaRue

Shaw

et al. 2005

J Virol

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The genomic structure and composition of an avian metapneumovirus (aMPV) recently isolated from wild Canada geese (goose 15a/01) in the United States, together with its replication, virulence, and immunogenicity in domestic turkeys, were investigated. The sizes of seven of the eight genes, sequence identity, and genome organization of goose aMPV were similar to those of turkey aMPV subtype C (aMPV/C) strains, indicating that it belonged to the subtype. However, the goose virus contained the largest attachment (G) gene of any pneumovirus or metapneumovirus, with the predicted G protein of 585 amino acids (aa) more than twice the sizes of G proteins from other subtype C viruses and human metapneumovirus and more than 170 aa larger than the G proteins from the other aMPV subtypes (subtypes A, B, and D). The large G gene resulted from a 1,015-nucleotide insertion at 18 nucleotides upstream of the termination signal of the turkey aMPV/C G gene. Three other aMPV isolates from Canada geese had similarly large G genes, whereas analysis of recent aMPV strains circulating in U.S. turkeys did not indicate the presence of the goose virus-like strain. In vitro, the goose virus replicated to levels (2 ؋ 10 5 to 5 ؋ 10 5 50% tissue culture infective dose) comparable to those produced by turkey aMPV/C strains. More importantly, the virus replicated efficiently in the upper respiratory tract of domestic turkeys but with no clinical signs in either day-old or 2-week-old turkeys. The virus was also horizontally transmitted to naïve birds, and turkey infections with goose 15a/01 induced production of aMPVspecific antibodies. Challenging day-old or 2-week-old turkeys vaccinated with live goose aMPV resulted in lower clinical scores in 33% of the birds, whereas the rest of the birds had no detectable clinical signs of the upper respiratory disease, suggesting that the mutant virus may be a safe and effective vaccine against aMPV infection outbreaks in commercial turkeys.

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confidence: 55%

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confidence: 99%

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A Wild Goose Metapneumovirus Containing a Large Attachment Glycoprotein Is Avirulent but Immunoprotective in Domestic Turkeys

Bennett

LaRue

Shaw

et al. 2005

J Virol

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“…The viruses used were P63-APV (Patnayak et al ., 2002) and ca-APV (Patnayak et al ., 2003). Both viruses were grown in the aforementioned cells and then titrated in homologous cells as well as in Vero cells.…”

Section: Methodsmentioning

confidence: 99%

“…In the US, two live, attenuated vaccines against subgroup C have been found to be safe and effective (Patnayak et al ., 2002(Patnayak et al ., , 2003. One of these vaccines was developed by serial propagation of virulent APV in cell cultures (Patnayak et al ., 2002) and the other by cold adaptation of APV (Patnayak et al ., 2003). These two vaccine strains have been designated as P63-APV and ca-APV, respectively.…”

Section: Introductionmentioning

confidence: 99%