Climate change could impose great influence on infrastructures. Previous studies have shown that solar radiation is one of the most important factors causing the change in temperature distribution in bridges. The current temperature distribution models developed in the past are mainly based on the meteorological data from the nearest weather station, empirical formulas, or the testing data from model tests. In this study, a five-span continuous Prestressed-concrete box-girder bridge was instrumented with pyranometers, anemometers, strain gauges, displacement gauges, and temperature sensors on the top and bottom slabs and webs to measure the solar radiation, wind speeds, strain, displacement, and surface temperatures, respectively. The continuously monitoring data between May 2019 and May 2020 was used to study the temperature distributions caused by solar radiation. A maximum positive lateral temperature gradient prediction model has been developed based on the solar radiation data analysis. Then, the solar radiation boundary condition obtained from the monitoring data and the lateral temperature gradient prediction model were utilized to compute the tensile stresses in the longitudinal and transverse directions. It was demonstrated in this study that the tensile stress caused by the lateral temperature gradient was so significant that it cannot be ignored in structural design.