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Our recent study reported that conformation change of granule-associated Bovine Serum Albumin (BSA) may influence the role of the protein controlling colloid deposition in porous media (Flynn et al., 2012). The present study conceptualized the observed phenomena with an ellipsoid morphology model, describing BSA as an ellipsoid taking a side-on or end-on A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT5 conformation on granular surface, and identified the following processes: (1) at low adsorbed concentrations, BSA exhibited a side-on conformation blocking colloid deposition; (2) at high adsorbed concentrations, BSA adapted to an end-on conformation promoted colloid deposition; (3) colloid deposition on the BSA layer may progressively generate end-on molecules (sites) by conformation change of side-on BSA, resulting in sustained increasing deposition rates. Generally, the protein layer lowered colloid attenuation by the porous medium, suggesting the overall effect of BSA was inhibitory at the experimental time scale.A mathematical model was developed to interpret the ripening curves. Modelling analysis identified the site generation efficiency of colloid as a control on the ripening rate (declining rate in colloid concentrations), and this efficiency was higher for BSA adsorbed from a more dilute BSA solution.
Our recent study reported that conformation change of granule-associated Bovine Serum Albumin (BSA) may influence the role of the protein controlling colloid deposition in porous media (Flynn et al., 2012). The present study conceptualized the observed phenomena with an ellipsoid morphology model, describing BSA as an ellipsoid taking a side-on or end-on A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT5 conformation on granular surface, and identified the following processes: (1) at low adsorbed concentrations, BSA exhibited a side-on conformation blocking colloid deposition; (2) at high adsorbed concentrations, BSA adapted to an end-on conformation promoted colloid deposition; (3) colloid deposition on the BSA layer may progressively generate end-on molecules (sites) by conformation change of side-on BSA, resulting in sustained increasing deposition rates. Generally, the protein layer lowered colloid attenuation by the porous medium, suggesting the overall effect of BSA was inhibitory at the experimental time scale.A mathematical model was developed to interpret the ripening curves. Modelling analysis identified the site generation efficiency of colloid as a control on the ripening rate (declining rate in colloid concentrations), and this efficiency was higher for BSA adsorbed from a more dilute BSA solution.
A multipurpose filter in which sand and clinoptilolite are used together as filter material is suggested for the simultaneous removal of suspended solids and ammonium in one single unit. The capacity of the clinoptilolite used was determined as 10.4 mg/g for 20 mg/l initial ammonium concentration. In addition, a packed column ion exchanger with clinoptilolite and a classical sand filter were also investigated for comparison. Ammonium and suspended solids removal rates were observed and compared for all columns. The results of the column analysis have revealed that the ammonium removal rate, which was only 20% in the sand column, was increased to 100% by replacing 50% of the sand with clinoptilolite; similarly, the suspended solids removal, which was only 17% in the clinoptilolite column, was increased to 75% by replacing 50% of the clinoptilolite with sand. As such, when ion exchange and filtration processes were carried out in a multipurpose column, high removals for both parameters could be obtained simultaneously. Multipurpose columns could be an alternative both for the upgrading of existing treatment plants or for new plants for simultaneous removal of ammonium and suspended solids, giving considerable savings in terms of land requirements.
Performance of a synthetic-fibre mat was investigated for the treatment of high-turbidity runoff. The impact of different operating parameters, such as hydraulics (flow rate and exchange rate), density current due to temperature and turbidity differences, mat conditions (thickness and number) and particle size were studied. The experimental results showed that increasing the mat thickness and number enhanced turbidity removal. The density current showed significant inverse effect on mat performance. Turbidity removal decreased with the increasing flow rate and exchange rate. Particle size also indicated an influence on removal efficiency. Predictive correlations for turbidity removal efficiency and mat design were established using dimensionless groups based on the experiment. The simulation results indicated that the predicted values correlated with the experimental ones significantly. Finally, the application design process was demonstrated.
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