Experimental and natural infection with Bartonella henselae in domestic cats

Abbott, Rachel C.; Chomel, Bruno B; Kasten, Rickie W.; Floyd-Hawkins, Kim; Kikuchi, Yoko; Koehler, Jane E.; Pedersen, Niels C

doi:10.1016/s0147-9571(96)00025-2

Cited by 163 publications

(174 citation statements)

References 23 publications

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“…Cats inoculated by the intradermal route with a pathogenic strain of B. henselae type II (LSU16) developed fever after 6 to 16 days PI, which lasted for 1 to 8 days [45]. Our present data were in contrast to all our previous results, using a B. henselae type II strain, that did not cause any fever or clinical signs [1,59]. These clinical observations may support strain dependent differences in the pathogenicity of B. henselae, as previously suggested [45,51].…”

Section: Discussioncontrasting

confidence: 99%

“…Blood (1.5 mL) was transferred into a pediatric lysis-centrifugation tube (Wampole Laboratories, Cranbury, NJ, USA) and centrifuged, and the pellet was plated onto 5% rabbit blood agar, as previously described [1]. The isolate was confirmed to be a B. henselae strain by the polymerase chain reaction (PCR) and determined to belong to B. henselae type I by 16S rRNA-DNA sequencing, as previously published [29].…”

Section: Primary Isolation Of B Henselae Feline Type Imentioning

confidence: 99%

“…The harvested colonies were suspended into sterile saline and 0.5 mL was inoculated intradermally in 3-5 different sites, as previously described [1]. For the B. henselae feline type I infection, all six cats were inoculated with 4.8 × 10 7 colony-forming units (CFU)/mL.…”

Section: Experimental Inoculationmentioning

confidence: 99%

“…Two milliliters of blood were placed into ethylenediamine tetraacetate (EDTA) tubes (Becton Dickinson, Franklin Lakes, NJ, USA) for culture and 1 mL into serum separation tubes for serological tests. The EDTA tubes were frozen at -70 o C and plated a few days later onto 5% rabbit blood agar plates, as previously described [1].…”

Section: Blood Culturementioning

confidence: 99%

“…Epidemiological and experimental studies have demonstrated that domestic cats represent the main reservoir of B. henselae [10,12,33,49,59] and B. henselae is primarily transmitted among cats by fleas (Ctenocephalides felis) [14,22,32]. Cats are persistently infected with Bartonella in their bloodstream for a few months up to several years [1,35,38]. Bartonella henselae have been classified in two predominant serotypes/genotypes, type I and type II, also designated as Houston I and BA-TF/Marseille based on 16S rRNA-DNA sequencing [6,7,20,31,53,55].…”

Section: Introductionmentioning

confidence: 99%

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Experimental infection of specific pathogen free (SPF) cats with two different strains of Bartonella henselae type I: A comparative study

et al. 2002

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-Domestic cats are the reservoir of Bartonella henselae, the main causative agent of cat scratch disease. We compared B. henselae type I infection characteristics in 6 SPF cats infected with a feline strain (4.8 × 10 7 colony-forming units (CFU)/mL) and in 6 SPF cats infected with the reference Houston I strain (6.6 × 10 6 CFU/mL to 9.6 × 10 7 /mL). All the cats inoculated with the feline strain, but none of the cats inoculated with B. henselae Houston I, developed a fever within 2-12 days (mean: 5.8 days) post inoculation (PI), which lasted for 1-2 weeks. However, all 12 cats became bacteremic. The duration of bacteremia was significantly longer in the cats inoculated with the feline strain (mean: 237 days) than in the cats inoculated with Houston I strain (mean: 60 days) (p < 0.01). Five (83%) cats inoculated with the feline strain and none of the six cats inoculated with B. henselae Houston I had relapsing bacteremia (p = 0.02). IgG antibodies were detected by IFA within 1-2 weeks for both strains, but peaked later (week 10 versus week 3 PI) for the feline strain. By ELISA, using antigens of each B. henselae strain, all 12 cats developed Bartonella specific IgM and IgG antibodies, but the cats infected with B. henselae Houston I antigen yielded significantly lower optical density values (p < 0.05). By SDS-PAGE, PFGE and Western blotting, protein profile differences (84 to 89% homology) were observed between the two strains. If a feline vaccine is to be developed in order to prevent human infection, the choice of the vaccine strain will be critical, since major differences were identified even between strains belonging to the same sero/genotype. Résumé -Étude comparative de l'infection expérimentale de chats sans germe pathogène spécifique avec deux souches différentes de Bartonella henselae type I. Le chat domestique constitue le réservoir de Bartonella henselae, l'agent principal de la maladie des griffes du chat. Les caractéristiques de l'infection expérimentale de chats domestiques sans germe pathogène spécifique par une souche féline de B. henselae type I (6 chats recevant une dose de 4,8 × 10 7 colonies/mL) ou de la souche de référence Houston I (6 chats recevant entre 6,6 × 10 6 et 9,6 × 10 7 colonies/mL) sont présen-tées. Les six chats infectés avec la souche féline, mais aucun des six chats infectés avec la souche Houston I, développèrent de la fièvre 2 à 12 jours après l'inoculation (moyenne : 5,8 jours) et celle-ci dura entre 1 et 2 semaines. Cependant, les 12 chats devinrent bactériémiques, mais la bactériémie dura significativement plus longtemps chez les chats infectés par la souche féline (moyenne : 237 jours) que chez les chats infectés par la souche Houston I (moyenne : 60 jours) (p < 0.01). La majorité (83 %) des chats inoculés avec la souche féline présentèrent des récurrences de bactériémie, alors qu'aucun des chats inoculés avec la souche Houston I n'en présenta (p < 0.02). Les anticorps IgG, détectés par immunofluorescence chez tous les chats entre 1 et 2 semaines après l'inocula...

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Section: Discussioncontrasting

confidence: 99%

Section: Primary Isolation Of B Henselae Feline Type Imentioning

confidence: 99%

Section: Experimental Inoculationmentioning

confidence: 99%

Section: Blood Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Experimental infection of specific pathogen free (SPF) cats with two different strains of Bartonella henselae type I: A comparative study

et al. 2002

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Bacterial Diseases

Weese

BSc

2010

Companion Animal Zoonoses

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Detection of Bartonella henselae in feline erythrocytes in Egypt by using Giemsa staining, transmission electron microscopy, and polymerase chain reaction

Alsaadawy,

Sayed,

Ali

et al. 2024

Microscopy Res & Technique

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Bartonella species (Bartonella spp.) have gained recognition as a significant human pathogen, implicated in a wide range of diseases. Among these, Bartonella henselae infection has been extensively studied for its primary occurrence in cats and its role in the development of cat‐scratch disease in humans. While light microscopy and transmission electron microscopy (TEM) have traditionally played crucial roles in identifying causative agents of infectious diseases, including Bartonella spp., the accuracy of these methods in identifying Bartonella spp. remains undefined. Therefore, this study aims to bridge this gap by employing both light microscopy and TEM to detect Bartonella in feline blood samples and to confirm B. henselae with polymerase chain reaction (PCR). Examination of blood smears stained with Giemsa and toluidine blue semithin sections by using light microscopy revealed the presence of intraerythrocytic corpuscles, suggesting Bartonella infection in six out of 33 examined cat blood samples. TEM findings corroborated these observations, showcasing the engulfment of bacteria by the erythrocyte membrane, along with the presence of some Bartonella spp., adhering to the erythrocyte wall. PCR‐based molecular detection confirmed the presence of B. henselae in these six samples. It is concluded that light microscopy and TEM are considered valuable in the screening of cats' blood for the potential presence of Bartonella. However, further molecular techniques are essential for precise identification and confirmation of specific Bartonella spp.Research Highlights Giemsa‐stained blood smear and semithin section showed potential intraerythrocytic Bartonella spp. corpuscles. TEM demonstrated the engulfment of Bartonella spp. by the erythrocyte membrane, along with the presence of some Bartonella spp. adhering to the erythrocyte wall. Molecular analysis of blood samples from cats by PCR unveiled that six out of 33 (18.18%) samples tested positive for B. henselae infection.

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Experimental and natural infection with Bartonella henselae in domestic cats

Cited by 163 publications

References 23 publications

Experimental infection of specific pathogen free (SPF) cats with two different strains of Bartonella henselae type I: A comparative study

Experimental infection of specific pathogen free (SPF) cats with two different strains of Bartonella henselae type I: A comparative study

Bacterial Diseases

Detection of Bartonella henselae in feline erythrocytes in Egypt by using Giemsa staining, transmission electron microscopy, and polymerase chain reaction

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