Saving energy while maintaining a high-quality internal environment is an increasingly important scientific and technological challenge in the building sector. This paper presents the results from a long-term study on thermal comfort in a passive house situated in the south of Poland. The building was constructed in 2010 with the use of prefabricated, lightweight technology. The main energy source is a ground source heat pump which powers the floor heating and DHW. The building is also equipped with a mechanical ventilation system with heat recovery and a ground source heat exchanger. A lightweight building structure which has active systems with limited capabilities (especially for cooling) is a combination which increases the difficulty of maintaining a proper inner environmental condition. Extensive experimental investigations on hygrothermal performance and energy use have been carried out in the building for several years. The measurement results, such as inner air temperature and humidity, as well as the inner surface temperature of partitions, could be directly used to determine basic thermal comfort indicators, including PMV and PPD. Any missing data that has not been directly measured, such as the surface temperature of the windows, floors, and some of the other elements of the building envelope, have been calculated using WUFI®PLUS software and validated with the available measurements. These results are not final; the full measurement of thermal comfort as an applied methodology did not consider human adaptation and assumed constant clothing insulation. Nevertheless, in general, the results show good thermal comfort conditions inside the building under research conditions. This was also confirmed via a survey of the inhabitants: 2 adults and 3 children.