Experimental Aspergillosis in buffaloes: Histopathological, histochemical and serological studies

Summary Thirty‐one (including ten lactating) healthy goats were divided into 3 groups viz. intravenous (I/V), intratracheal (I/T) and intramammary (I/M) on the basis of route of inoculation of Aspergillus fumigatus spores. Two animals were kept as control for each group. Clinical aspergillosis developed and was more severe and fatal in consequence in I/V group than those of other groups. Respiratory symptoms appeared and persisted in both I/V and I/T groups. Acute mastitis developed in the right infected udder halves only with a brief and transitory illness in all goats of I/M group and marked discrepancy in size between right inoculated and left uninfected udder halves was observed. Haematological examination revealed significant decrease of haemoglobin concentration and significant increase in both TLC and DLC (neutrophilia) in I/V group but no marked haematological change was observed in I/T and I/M groups. Mycological isolation in pure culture, demonstration of fungus in the impression smears by lactophenol cotton blue staining and in histological sections by PAS and GSM methods were achieved in the lungs, kidneys, heart, liver, spleen and abomasum in I/V group, in the lungs and occasionally in the mediastinal lymph node and liver in I/T group and in the right infected udder halves and their milk/secretions in I/M group.

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An analysis of antibodies against Aspergillus jumigatus in bovine serum by iminunoblotting and enzyme‐linked immunosorbent assays

Jensen

Latgé

1995

APMIS

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Serum samples from 20 cows with spontaneously acquired systemic aspergillosis and serum collected consecutively from a cow with experimentally induced aspergillosis were assayed for antibodies against Aspergillus fumigatus in immunoblotting and enzyme‐linked immunosorbent assays. Purified antigens (18 kDa protein and 20 kDa galactomannan) from A. fumigatus as well as complex preparations of metabolic and somatic antigens were used. Antibodies against A. fumigatus were found in both infected and non‐infected cows. High titers and especially an increase in titer in consecutive samples signified infection. Reactivity with the 22 and 32 kDa antigens in preparations of exocellular antigens and the 19, 20, and 39 kDa antigens from intracellular antigens was seen with sera from infected cows only, and is therefore indicative of infection.

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Experimental Aspergillosis in buffaloes: Histopathological, histochemical and serological studies

Cited by 3 publications

References 6 publications

Hexagonal clustering of intramembrane particles in the plasmalemma of Ustilago avenae after fungicide treatment

Hexagonal clustering of intramembrane particles in the plasmalemma of Ustilago avenae after fungicide treatment

Experimental Aspergillosis in Goats: Clinical, Haematological and Mycological Studies

An analysis of antibodies against Aspergillus jumigatus in bovine serum by iminunoblotting and enzyme‐linked immunosorbent assays

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