Alcohols have been known as influential alternatives for the utilization in the compression-ignition (CI) engines. In contrast to lower-order alcohols such as methanol-C1 and ethanol-C2, long-chain alcohols (higher-order alcohols) have a hopeful future for CI engines. Pentanol-C5 or amyl alcohol, regarding its affirmative chemical and physical properties, is a type of higher alcohol that can be obtained from biomass resources and hence it has to be evaluated as an alternating and sustainable fuel candidate in diesel engine applications. The objective of this work is to explore the engine performance and exhaust emission characteristics of a CI engine running on 1-pentanol/diesel fuel mixtures. For this aim of the experimental research, three different blends were created by infusing various ratios (10, 20, and 30% by volume) of 1-pentanol into pure diesel with implementing the splash-blending method to acquire the binary blends of Pt10, Pt20, and Pt30. The tested fuel samples were used in a single-cylinder diesel engine coupled with a generator. The influences of a next-generation alcohol addition to the diesel upon the engine performance along with exhaust emission levels of the tested engine were meticulously researched at six different engine loads (0, 0.4, 0.8, 1.2, 1.6, and 2 kW) with a stable speed (3000 rpm). The infusion of alcohol into the diesel fuel declined cetane number as well as the lower calorific value of the fuel blends. As a result of the study carried out, it was observed that the brake specific fuel consumption (BSFC) increased between 4.46-11.78% averagely as the ratio of 1-pentanol in the mixtures increased while brake thermal efficiency (BTE) and exhaust gas temperature (EGT) dropped up to 6.75% and 6.69%, respectively owing to the lesser energy content of the 1pentanol. When the test engine operating with binary blends, unburned hydrocarbon (HC) and carbon monoxide (CO) emissions were obtained to be higher than that of conventional diesel fuel due to the higher latent heat of vaporization (LHV) of 1-pentanol resulting in a cooling impact in the cylinder, leading descending trend in the efficiency of the combustion. Besides, the addition of 1pentanol to diesel caused the mitigation in smoke emission by 77.37-89.60%, carbon dioxide (CO2) by 13.06-30.83%, and nitrogen oxides (NOX) by 13.43-41.61% on an average as compared to diesel fuel. Overall, it has been shown up that 1-pentanol might be successfully utilized as an oxygenated fuel additive to diesel fuel, however in a minimum concentration of 1-pentanol, i.e., Pt10 blend has provided luminous outcomes in terms of mitigating the EGT, smoke opacity, and especiallyNOXemissions, however at the expense of boosting in the emissions of CO and HC.