Experimental assessment of the biosafety of Trissolcus japonicus in New Zealand, prior to the anticipated arrival of the invasive pest Halyomorpha halys

Charles, J. G.; Avila, Gonzalo A.; Hoelmer, Kim A.; Hunt, Sophia K.; Gardner-Gee, Robin; MacDonald, F.H.; Davis, V.A.

doi:10.1007/s10526-019-09949-x

Cited by 44 publications

(54 citation statements)

References 35 publications

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“…Some of these stink bugs are very common in northern Italy [59,60], where T. mitsukurii is likely to attack their eggs. Thus, although T. japonicus's fundamental host range has been already investigated [61][62][63], similar studies are needed for T. mitsukurii.…”

Section: Discussionmentioning

confidence: 99%

An Insight into the Role of Trissolcus mitsukurii as Biological Control Agent of Halyomorpha halys in Northeastern Italy

Scaccini

Falagiarda

Tortorici

et al. 2020

Insects

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Sustainable strategies such as classical or augmentative biological control are currently being evaluated for the long-term management of the alien invasive pest Halyomorpha halys (Stål) (Hemiptera: Pentatomidae). A three-year study carried out in northeastern Italy was performed to investigate the distribution and field performance of the H. halys egg parasitoid Trissolcus mitsukurii (Ashmead) (Hymenoptera: Scelionidae), in comparison with other parasitoid species. In the study area, adventive populations of T. mitsukurii were present since 2016, representing the earliest detection of this species in Europe. Trissolcus mitsukurii was the most abundant parasitoid and showed a higher “parasitoid impact” (i.e., number of parasitized eggs over the total number of field-collected eggs) compared to the other species, i.e., Anastatus bifasciatus (Geoffroy) (Hymenoptera: Eupelmidae), Trissolcus basalis (Wollaston) and Trissolcus kozlovi Rjachovskij (Hymenoptera: Scelionidae). The hyperparasitoid Acroclisoides sinicus (Huang and Liao) (Hymenoptera: Pteromalidae) was also recorded. Phylogenetic analysis of T. mitsukurii population distinguished two clades, one covering samples from Italy, Japan and China, the other from South Korea. The present study provides promising results for the biological control of a pest that is having a dramatic impact on a wide range of crops worldwide.

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Section: Discussionmentioning

confidence: 99%

An Insight into the Role of Trissolcus mitsukurii as Biological Control Agent of Halyomorpha halys in Northeastern Italy

Scaccini

Falagiarda

Tortorici

et al. 2020

Insects

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“…In fact, laboratory host range studies on a closely related species, Nezara viridula (both species are in the tribe Nezarini), found similar levels of unemerged nymphs (approx. 50%) in egg masses that were exposed to T. japonicus [ 43 , 44 ], and unexposed, control egg masses of Nezara viridula also had approximately 50% unemerged eggs [ 44 , 45 ], indicating that the high levels of mortality in this group may be unrelated to parasitoid exposure. However, unemerged eggs could also, at least in principle, be as a result of unsuccessful oviposition by a parasitoid (i.e., probing) that does not leave DNA evidence.…”

Section: Discussionmentioning

confidence: 99%

Hidden Host Mortality from an Introduced Parasitoid: Conventional and Molecular Evaluation of Non-Target Risk

Hepler

Athey

Enicks

et al. 2020

Insects

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Hidden trophic interactions are important in understanding food web ecology and evaluating the ecological risks and benefits associated with the introduction of exotic natural enemies in classical biological control programs. Although non-target risk is typically evaluated based on evidence of successful parasitism, parasitoid-induced host mortality not resulting in visible evidence of parasitism (i.e., nonreproductive effects) is often overlooked. The adventive establishment of Trissolcus japonicus, an exotic parasitoid of the introduced stink bug Halyomorpha halys, provides an opportunity to investigate the total impact of this parasitoid on target and non-target hosts in the field. We developed a new methodology to measure nonreproductive effects in this system, involving a species-specific diagnostic PCR assay for T. japonicus. We applied this methodology to field-deployed eggs of four pentatomid species, coupled with traditional rearing techniques. Nonreproductive effects were responsible for the mortality of an additional 5.6% of H. halys eggs due to T. japonicus, and were even more substantial in some of the non-target species (5.4–43.2%). The observed hidden mortality of native non-target species from an introduced parasitoid could change predictions about direct and indirect ecological interactions and the efficacy of biological control of the target pest.

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“…Worldwide, T. japonicus is being considered or has already been studied as a biological control agent; this includes its use in classical biological control as well as redistribution efforts with inoculative and inundative releases (Jentsch 2017, Charles et al 2019Lowenstein et al 2020). These activities require large numbers of T. japonicus adults produced over short periods in order to release meaningful numbers of parasitoids throughout a growing season.…”

Section: Applications For T Japonicus Rearing To Support Releasesmentioning

confidence: 99%

An effective cold storage method for stockpilingHalyomorpha halyseggs for field surveys and laboratory rearing ofTrissolcus japonicus

Wong

Walz

Oscienny

et al. 2020

Preprint

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An effective stockpiling method for egg masses of the invasive brown marmorated stink bug (Halyomorpha halys [Stål]; Hemiptera: Pentatomidae) would be useful for rearing and field studies of its egg parasitoid Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae). The current method of stockpiling H. halys egg masses at sub-zero temperatures has lethal and sublethal fitness consequences for T. japonicus. We show that parasitoid emergence from egg masses refrigerated at 8°C for up to two months before parasitism is higher than from frozen egg masses and usually has minimal or no sublethal fitness effects (sex ratio, development time, activity, fecundity, longevity, and weight) on emerging T. japonicus. Only after two months of host egg refrigeration did the emergence of T. japonicus begin to decrease significantly (by 9.6% relative to untreated viable egg masses), whereas egg masses previously frozen at -80°C had a 58.8% reduction in parasitoid emergence after 14 days of storage. Refrigerated egg masses that were subsequently exposed to average field temperatures (warm: 22.9°C; cool: 13.2°C) were still suitable for T. japonicus parasitism after 7 days, while viable egg masses exposed to warm temperatures for 7 days before parasitism had 24.1% lower parasitoid emergence. Our results demonstrate that refrigeration at 8°C, while resulting in complete mortality of H. halys embryos after 10 days, are more suitable for T. japonicus parasitism than those stored at sub-zero temperatures. The quantity and quality of H. halys eggs that can be stockpiled with this method could facilitate T. japonicus laboratory colony maintenance, field monitoring, and releases.

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Experimental assessment of the biosafety of Trissolcus japonicus in New Zealand, prior to the anticipated arrival of the invasive pest Halyomorpha halys

Cited by 44 publications

References 35 publications

An Insight into the Role of Trissolcus mitsukurii as Biological Control Agent of Halyomorpha halys in Northeastern Italy

An Insight into the Role of Trissolcus mitsukurii as Biological Control Agent of Halyomorpha halys in Northeastern Italy

Hidden Host Mortality from an Introduced Parasitoid: Conventional and Molecular Evaluation of Non-Target Risk

An effective cold storage method for stockpilingHalyomorpha halyseggs for field surveys and laboratory rearing ofTrissolcus japonicus

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