Experimental autoimmune anterior uveitis (EAAU). II. Dose-dependent induction and adoptive transfer using a melanin-bound antigen of the retinal pigment epithelium

Broekhuyse, R.M.; Kuhlmann, E.D.; Winkens, H.J.

doi:10.1016/0014-4835(92)90112-6

Cited by 57 publications

(43 citation statements)

References 18 publications

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“…The determinations of soluble, membrane-, and melanin-protein, SDS-PAGE, the check for purity by SDS-PAGE and immunoblotting have been described previously (Broekhuyse et al, 1991(Broekhuyse et al, , 1992a(Broekhuyse et al, , b, 1993.…”

Section: Antigensmentioning

confidence: 99%

Differential Effect of Macrophage Depletion on Two Forms of Experimental Uveitis Evoked by Pigment Epithelial Membrane Protein (EAPU), and by Melanin-Protein (EMIU)

Broekhuyse

Huitinga

Kuhlmann

et al. 1997

Experimental Eye Research

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The purpose of the present study was to clinically and histologically investigate the influence of macrophage depletion on the development of experimental autoimmune pigment epithelial membrane protein-induced uveitis (EAPU), and experimental melanin-protein induced uveitis (EMIU) in the Lewis rat. EAPU is mainly characterized by pigment epitheliitis. Posterior mononuclear cell accumulations enclose and destroy the retinal pigment epithelium (RPE). In EMIU the inflammation is specifically localized in the uvea.EAPU was induced by immunization with RPE membrane protein, and EMIU was evoked by immunization with purified choroidal melanin. Systemic treatment with dichloromethylene diphosphonate (Cl2MDP)-containing liposomes just before the expected beginning of the clinical signs of EAPU (at day 7 and 9 after immunization) resulted in a considerable delay of the uveitis process. In the treated animals the typical plaque shaped cell accumulations (containing many macrophages) along the RPE were lacking. Two weeks after the treatment, severe rebound EAPU developed. Local treatment by subconjunctival liposome injections did not exert any effect on EAPU. In EMIU, macrophage depletion by systemic treatment did not noticeably influence the clinical and histological development of the inflammation.Systemic treatment at the peak stage of EAPU (at day 12 and 14 after immunization) resulted in the rapid disappearance of the clinical signs of uveitis. Vitreous and anterior chamber cells were virtually absent two days later. This situation remained unchanged until the experiment was terminated two weeks later. Already deposited cell accumulations along the RPE did not regress but stopped their progression.Hematogenous macrophages thus appear to play a crucial role in the development of EAPU but the effect of early macrophage depletion on EAPU appeared to be temporary due to blood repopulation, A possible explanation for the differential influence of macrophage depletion on EAPU and EMIU is discussed, and is based on differences in immunopathogenesis, © 1997 Academic Press Limited Key words: experimental autoimmune pigment epithelial membrane protein-induced uveitis (EAPU); experimental melanin-protein induced uveitis (EMIU); macrophages; uvea; retinal pigment epithelium; pigment epithelial membrane protein; dichloromethylene diphosphonate (C12MDP). 1* IntroductionThree markedly different types of experimental auto immune uveitis have been described which can. be

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Section: Antigensmentioning

confidence: 99%

Differential Effect of Macrophage Depletion on Two Forms of Experimental Uveitis Evoked by Pigment Epithelial Membrane Protein (EAPU), and by Melanin-Protein (EMIU)

Broekhuyse

Huitinga

Kuhlmann

et al. 1997

Experimental Eye Research

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“…The recurrent nature of autoimmune uveitis may result in severe clinical complications, such as cystoid macular edema, cataract formation, and glaucoma, even though a single episode of the disease usually does not cause permanent visual loss. Experimental autoimmune uveitis (EAU) 3 can be elicited in rodents following immunization with several different Ags, e.g., retinal-soluble Ag, interphotoreceptor retinal-binding protein (IRBP) (1, 2), melanin-associated Ag (MAA) (3,4), and myelin proteins (5-7), as well as by the adoptive transfer of uveitogenic T cells between syngeneic rodents (7)(8)(9)(10). Uveitogenic T cells that mediate EAU are assumed to be exclusively CD4 ϩ T cells.…”

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confidence: 99%

Conversion of Monophasic to Recurrent Autoimmune Disease by Autoreactive T Cell Subsets

Shao

Song

Sun

et al. 2003

The Journal of Immunology

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Autoimmune uveitis has been elicited in susceptible rodents by several ocular-specific Ags. In most of these animal models the induced uveitis is acute and monophasic. Because recurrent uveitis poses the highest risk for blinding ocular complications in human disease, a spontaneous relapsing animal model would be most helpful in understanding the disease pathogenesis. In our current study we have observed that the adoptive transfer of interphotoreceptor retinoid-binding protein residues 1177–1191-specific T cells to naive Lewis rats induced a chronic relapsing disease, in contrast to the monophasic disease induced by immunization with interphotoreceptor retinoid-binding protein residues 1177–1191 emulsified in CFA. The chronic relapsing uveitis induced by autoreactive T cell subsets is dependent on the number of autoreactive T cells generated as well as their activation status. Our study documented a spontaneous model of recurrent uveitis in the rat, which should assist us in the study of disease pathogenesis and the design of specific therapy.

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“…Non-soluble melanin proteins isolated from pigmented bovine eye tissues can induce an uveitis model named EMIU (50,51) . According to Matteson et al (52) , this model can be induced by subcutaneous injection of melanin proteins in association with Hunter adjuvant.…”

Section: Experimental Melanin Protein-induced Uveitis (Emiu)mentioning

confidence: 99%

Experimental models of autoimmune inflammatory ocular diseases

Gasparin¹,

Takahashi²,

Scolari³

et al. 2012

Arq. Bras. Oftalmol.

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Experimental autoimmune anterior uveitis (EAAU). II. Dose-dependent induction and adoptive transfer using a melanin-bound antigen of the retinal pigment epithelium

Cited by 57 publications

References 18 publications

Differential Effect of Macrophage Depletion on Two Forms of Experimental Uveitis Evoked by Pigment Epithelial Membrane Protein (EAPU), and by Melanin-Protein (EMIU)

Differential Effect of Macrophage Depletion on Two Forms of Experimental Uveitis Evoked by Pigment Epithelial Membrane Protein (EAPU), and by Melanin-Protein (EMIU)

Conversion of Monophasic to Recurrent Autoimmune Disease by Autoreactive T Cell Subsets

Experimental models of autoimmune inflammatory ocular diseases

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