Experimental canine leishmaniasis: Clinical, parasitological and serological follow-up

Maia, Carla; Nunes, Mónica; Cristóvão, José Manuel; Campino, Lenea

doi:10.1016/j.actatropica.2010.08.001

Cited by 47 publications

(44 citation statements)

References 30 publications

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“…A study of 12 dogs experimentally infected with L. infantum followed over a period of six months showed that all dogs became positive within four months after infection. The dogs presented with clinical symptoms such as lymphadenopathy and lesions of skin 29 , which are similar to the results of this study in which dogs were naturally infected.…”

Section: Discussionsupporting

confidence: 79%

THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi

Alves¹,

Silva²,

Batista

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThis study investigated the sero-conversion period in which dogs from endemic areas test positive for visceral leishmaniasis (VL) as well as the early post-infection period in which renal alterations are observed. Dogs that were initially negative for Canine Visceral Leishmaniasis (CVL) were clinically evaluated every three months by serological, parasitological and biochemical tests until seroconversion was confirmed, and six months later a subsequent evaluation was performed. Samples of kidney tissues were processed and stained with Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS) and Masson's trichrome stain and lesions were classified based on the WHO criteria. Of the 40 dogs that initially tested negative for VL, 25 (62.5%) exhibited positive serological tests during the study period. Of these 25 dogs, 15 (60%) tested positive within three months, five (20%) tested positive within six months and five (20%) tested positive within nine months. The dogs exhibited antibody titers between 1:40 and 1:80 and 72% of the dogs exhibited clinical symptoms. The Leishmania antigen was present in the kidneys of recently infected dogs. We found higher levels of total protein and globulin as well as lower levels of albumin in the infected dogs when compared to the control dogs. Additionally, infected dogs presented levels of urea and creatinine that were higher than those of the uninfected dogs. Glomerulonephritis was detected in some of the dogs examined in this study. These data suggest that in Teresina, the sero-conversion for VL occurs quickly and showed that the infected dogs presented abnormal serum proteins, as well as structural and functional alterations in the kidneys during the early post-infection period.

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Section: Discussionsupporting

confidence: 79%

THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi

Alves¹,

Silva²,

Batista

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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“…1). Seroconversion occurred between D120 and D180, similar to that noted in other trials Rodríguez-Cortés et al, 2007), while others have reported seroconversion on D30-45 (Leandro et al, 2001;Maia et al, 2010). On D360, all dogs showed higher antibody titers (range 1/3200-1/12,800) than those found in other experimental infections, where the method's detection limit was over 1/400 (Rosypal et al, 2005) or 1/640 .…”

Section: Resultssupporting

confidence: 73%

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

Hernández

Montoya

Checa

et al. 2015

Veterinary Parasitology

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“…We therefore chose to use metacyclic promastigotes derived from amastigotes after the minimum number of amplification passages to obtain the required number of parasites. A more recent study using intravenous administration of a very similar number of parasites in the amastigote form, did not appear to lead to a more rapid progression to active infection than that observed in the current study [48]. …”

Section: Discussioncontrasting

confidence: 59%

The protective immune response produced in dogs after primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) remains effective against an experimental challenge one year later

Martin

Vouldoukis

Moreno

et al. 2014

Vet Res

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Control of canine leishmaniasis is an important objective for the benefit of dogs living in or visiting endemic areas and for public health because of the zoonotic nature of this disease. Resistance or susceptibility to developing canine leishmaniasis after exposure to Leishmania infantum is primarily determined by the ability of the immune system to develop an appropriate Th1-dominated specific response to the parasite. For this reason there is a need for effective canine vaccines that can decrease the number of dogs developing progressive infections. In this study, we followed the impact of the LiESP/QA-21 canine vaccine (composed of excreted-secreted proteins of L. infantum and the QA-21 saponin adjuvant), recently launched commercially in Europe, on selected humoral and cellular immune parameters following an infectious intravenous challenge with L. infantum promastigotes administered one year after the primary vaccine course. We also followed parasitological parameters to determine the parasitological status of the challenged dogs. In contrast to controls, vaccinated dogs retained significantly stronger cell-mediated immune responses against the parasite despite a virulent challenge and had significantly lower mean parasite burdens at the end of the study, associated with a lower probability of developing active infections. These results confirm that the immune responses generated by vaccination with LiESP/QA-21 are still effective against an intravenous challenge one year after the primary vaccine course.

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Experimental canine leishmaniasis: Clinical, parasitological and serological follow-up

Cited by 47 publications

References 30 publications

THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi

THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

The protective immune response produced in dogs after primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) remains effective against an experimental challenge one year later

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