The effect of different conditioner doses and different applied pressures on the dewaterability of municipal sludge during the filtration and expression stage was assessed using a series of experiments referred to as a uniform design. The relative importance of conditioner dose and applied pressure on the dewatering characteristics of municipal sludge was evaluated with a quadratic model using partial least squares (PLS) regression analysis. It was shown that the developed quadratic model was able to accurately predict the dewatering characteristics during the whole process. It was further shown that lime as a physical conditioner had the most significant impact on filtration rate, expression rate, cake dry solids content, net sludge solids yield, and total cycle time. Aluminum polychloride as the inorganic flocculant had a quite complex effect although not as significant as lime. Filtration pressure had only a slight effect on dewaterability of the municipal sludge, though higher feeding pressure was needed in order to enhance the total feeding amount. Furthermore, expression pressure was only of benefit to cake dry solids content and expression rate and failed to improve other dewatering characteristics of the municipal sludge.