“…In contrast with linear electroosmotic streaming on insulating charged channel sidewalls, EHD fluid motion appears as a series of flow vortex above an array of ideally polarizable metal-strip electrodes, and the voltage required is commonly no more than dozens of volts [16,17]. In this way, AC nonlinear electrokinetics is able to achieve a higher degree of freedom control on localized flow behavior, and finds interesting applications in pumping, mixing, and separation of target analyte in the context of microfluidics, in virtue of its superior flexibility by adjusting the amplitude, phase gradient, and field frequencies of the voltage wave, namely, AC electrothermal (ACET) [18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38] and AC electroosmosis (ACEO) [39,40,41,42,43,44,45,46,47,48,49,50,51,52,53].…”