Experimental Chronic Pulmonary Infection in Mice Caused by <i>Klebsiella pneumoniae</i>

Iizawa, Yuji; Nishi, Takeshi; Kondo, Masahiro; Imada, Akira

doi:10.1111/j.1348-0421.1988.tb01451.x

Cited by 11 publications

(9 citation statements)

References 15 publications

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“…Initially, authors [6] described bronchopneumonia in rats, by intranasal inoculation of bacteria, but they eventually abandoned their model due to high mortality in the early phase of infection. Chronic pulmonary infection in mice by K. pneumoniae has so far been reported only by researchers [7]. These workers observed a marked variation in susceptibility of a mouse strain to an experimental respiratory tract infection, besides high mortality towards the later part of observation period.…”

Section: Discussionmentioning

confidence: 93%

Occurrence of SHV GENE and Antibiogram of Klebsiella Pneumoniae Infection in Swiss Albino Mice Colony

Thangapandiyan

Preetha

Kannan

et al. 2016

IRA-JAS

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Section: Discussionmentioning

confidence: 93%

Occurrence of SHV GENE and Antibiogram of Klebsiella Pneumoniae Infection in Swiss Albino Mice Colony

Thangapandiyan

Preetha

Kannan

et al. 2016

IRA-JAS

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“…Animals. We used two strains of mice: 5-week-old Crj:CDF 1 female mice and CBA/JNCrj female mice (Charles River Japan, Inc., Kanagawa, Japan) weighing 16 to 22 g. Since it is well known that there is a strain difference in susceptibility to an experimental infection (7,14,31), we established two types of infection models with the two strains of mice. Indeed, the response to pulmonary aspergillosis was quite different in the two strains.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of TAK-457, a Novel Intravenous Triazole, against Invasive Pulmonary Aspergillosis in Neutropenic Mice

Hayashi

Kitamoto

Iizawa

et al. 2002

Antimicrob Agents Chemother

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On the other hand, amphotericin B at 1 mg/kg which was a maximum tolerable dose showed slight but not significant prolongation of survival time of mice, although it also reduced the lung chitin levels and the plasma ␤-D-glucan levels to a lower extent but still significantly. These results suggest that TAK-457 is a promising candidate for development for the treatment of invasive aspergillosis in humans.

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“…To produce the chronic respiratory tract infection, mice were infected with K. pneumoniae 27 by aerosol exposure as described previously (11). In brief, mice were placed in an exposure chamber, and the bacterial suspension was aerosolized at a pressure of 1.2 kg/cm2 for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…The suspension of K. pneumoniae (biotype aerogenes, capsular type 1) for infection was prepared as described previously (11). Pseudomonas aeruginosa P9 with type G O-antigen, which was supplied by J.Y.…”

Section: Methodsmentioning

confidence: 99%

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Examination of Host Defense Factors Responsible for Experimental Chronic Respiratory Tract Infection Caused by Klebsiella pneumoniae in Mice

Iizawa

Nishi

Kondo

et al. 1991

Microbiology and Immunology

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We have studied the host defense factors that operate during the course of chronic respiratory tract infection caused by Klebsiella pneumoniae 27 in CBA/J mice. A large number of polymorphonuclear leukocytes (PMNs) rapidly infiltrated the alveolar spaces after infection.Treatment with cyclophosphamide (CY) before infection greatly reduced the infiltration of PMNs and caused an increase in bacterial counts.CY treatment of mice in the chronic phase also caused bacterial proliferation in the lungs. The administration of a high titer immune serum efficiently reduced the bacterial counts in the lungs during the early phase but not during the chronic phase.The proliferation of bacteria induced by CY treatment was not suppressed by the administration of the immune serum in either phase. When the mice were exposed to an aerosol containing Pseudomonas aeruginosa P9 in the chronic phase, the organisms from the secondary infection were eliminated from the lungs in the same manner as in the case of primary infection with P. aeruginosa. Thus, PMNs seem to play an important role in the suppression of bacterial proliferation in the early and chronic phases, and the specific antibody might have a supplementary effect on the defensive action of PMNs in the chronic phase.It is also presumed that the bacteria in the chronic phase of infection are sequestered at sites hardly accessible to PMNs.Although many antimicrobial agents are now available, chronic infections are still difficult to cure. Chronic infection models are therefore required to examine the host-parasite relationship in the chronic phase.We have previously established a model of chronic respiratory tract infection in mice caused by Klebsiella pneumoniae (11). The course of infection in this model is highly reproducible, and the bacterial counts in the lungs after infection change with time, showing four different phases. In the first phase, the bacterial counts decrease for 4 days. In the second phase, the bacterial counts increase starting day 4 and reaching the initial level 1 week after infection. The third phase lasts from 1 week to 4 weeks after infection, during which time the bacterial counts remain relatively unchanged. The fourth phase starts 4 weeks after infection, and thereafter the bacterial counts gradually increase. This model is useful for analyzing 615

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Experimental Chronic Pulmonary Infection in Mice Caused by Klebsiella pneumoniae

Abstract: Examination of mouse strain

Cited by 11 publications

References 15 publications

Occurrence of SHV GENE and Antibiogram of Klebsiella Pneumoniae Infection in Swiss Albino Mice Colony

Occurrence of SHV GENE and Antibiogram of Klebsiella Pneumoniae Infection in Swiss Albino Mice Colony

Efficacy of TAK-457, a Novel Intravenous Triazole, against Invasive Pulmonary Aspergillosis in Neutropenic Mice

Examination of Host Defense Factors Responsible for Experimental Chronic Respiratory Tract Infection Caused by Klebsiella pneumoniae in Mice

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