It was shown that
flavor chemicals with high toxicity sensitivities
mean that small changes in their effective concentrations can lead
to significant changes in toxicity. Flavors are widely used in personal
care products. However, our study demonstrated that some flavor chemicals
and their mixture rays have high toxicity sensitivities to
Caenorhabditis elegans
(
C. elegans
), which may have an impact on human health. In this paper, three
flavor chemicals (benzyl alcohol, phenethyl alcohol, and cinnamaldehyde)
were used as components of the mixture, and three binary mixture systems
were constructed, respectively. Five mixture rays were designed for
each mixture system by a direct equipartition ray design method. The
lethal toxicities of the three flavor chemicals and mixture rays to
C. elegans
at three exposure volumes were determined.
A new concept (inverse of the negative logarithmic concentration span
(iSPAN)) was introduced to quantitatively evaluate the toxicity sensitivity
of chemicals or mixture rays, and the combination index (CI) was employed
to identify the toxicological interactions in the mixtures. It was
shown that the three flavor chemicals as well as the binary mixture
rays have a significant concentration–response relationship
on the lethality of
C. elegans
. The
iSPAN values of the three flavor chemicals and their mixture rays
were larger than 3.000, showing very strong toxicity sensitivity to
C. elegans
. In mixture systems, the toxicity sensitivities
of mixture rays with different mixture ratios were also different
at different exposure volumes. In addition, it can be seen from the
CI heat map that the toxicological interaction not only shows the
mixture ratio dependence but also changes with the different exposure
volumes, which implies that the mixtures consisting of flavor chemicals
with high toxicity sensitivity have complex toxicological interactions.
Therefore, in environmental risk assessment, special attention should
be paid to chemicals with high toxicity sensitivities.