As an additive for diesel engines, copper oxide nanoparticles (CuONPs) are thought to offer a lot of promise. In this work, an experimental investigation was conducted to ascertain the impact on a diesel engine of adding copper oxide (CuO) particles made from a Rubus fruticosus leaf extract product using a novel green synthesis approach. The presence and morphology of the CuO were analyzed by different characterization studies. Subsequently, was investigated the effects of the addition of CuONPs at two concentrations (15 and 20 ppm) on diesel engine performance, brake‐specific fuel consumption (BSFC), carbon monoxide (CO), hydrocarbon (HC), nitrogen oxide (NOX), and smoke emissions. From the experimental outcomes, the D100 + 20 ppm CuO blend shows a significant reduction in CO, HC, and smoke emissions by 58.19%, 25.15%, and 26.67%, respectively, when compared with standard diesel. Also, it was found that the D100 + 20 ppm CuO blend showed better engine performance with enhanced combustion characteristics. The experimental results provided a reduction of around 1.10% in BSFC. This study demonstrates how changes in fuel composition through the addition of nanoparticles to commonly used diesel fuel can provide meaningful improvements in engine performance and emissions.