Experimental Evaluation of Tranexamic Acid–Loaded Porous Starch as a Hemostatic Powder

Abstract

We evaluated the effectiveness of a novel hemostatic powder called Tranexamic Acid-loaded Porous Starch (TAPS) developed recently on blood clotting activity and hemostasis. The effectiveness of TAPS was evaluated by comparing hemostatic properties with those of Quick-acting Styptic Powder (QSP) and Compound Microporous Polysaccharide Haemostatic powder (CMPHP). The blood clotting activities of human blood were analyzed by thromboela-stogram (TEG) assays in vitro. The hemostatic effectiveness in vivo was evaluated using a rat model with hepatic traumatic hemorrhage. The blood loss and standardized bleeding score, which reflects the degree of bleeding after treatment with styptic powder, were used to evaluate hemostatic efficacy. In vitro, the values of TEG parameters in TAPS group were significantly different, compared with untreated controls or CMPHP group (p < 0.05). In vivo, the application of QSP, CMPHP and TAPS led to significantly decreased post-treatment blood loss than in the control group (p < 0.01). The scores of the groups treated with QSP, CMPHP and TAPS (0, 0.2±0.422, 0.3±0.483, respectively) were significant lower than with gauze control (1.6±0.516) which success hemostatic was achieved at 5 minutes (p < 0.01). Hemostasis was achieved successfully within approximately 4 minutes after the application of TAPS. TAPS could help blood to form an artificial scab on a wound and to seal injuries for hemostasis to reduce blood loss in rats with hepatic trauma and hemorrhage. It was safe to use with no impact on blood clotting function or other apparent side effects.

Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

“…Additionally, chitosan acts as a blood coagulant, promoting platelet adhesion and aggregation. Additionally, its positively charged groups interact with the negatively charged red blood cell membranes [91]. Incorporation of NC-GL resulted in a significant (p < 0.05) reduction in OD values for scaffolds with a 6% w/v total polymer content.…”

A Bioinspired Hemostatic Powder Derived from the Skin Secretion ofAndrias davidianusfor Rapid Hemostasis and Intraoral Wound Healing

“…After collection, SSAD was washed with sterile phosphate-buffered saline (PBS), shaken, and certificated. Then, the SSAD solution was freeze-dried for 24 h, and grinded into powders of different particle sizes (4,8,14,20,60,100,200, and 300 meshes), which were additionally refined by straining through size-matched sieves. The SSAD powders were stored under −20 °C until further use.…”

Use of hemostatic agents for surgical bleeding in laparoscopic partial nephrectomy: Biomaterials perspective

“…MPH, produced from plat‐derived starch, is an absorbable HA introduced for the first time by Hursey and Dechene, which is now gaining popularity for use in surgery (Hursey & Dechene, 1989; Xi et al, 2018). The particular porous surface and hydrophilic nature of MPHs enable rapid absorption of water and low molecular weight compounds from the blood upon application.…”

“…The particular porous surface and hydrophilic nature of MPHs enable rapid absorption of water and low molecular weight compounds from the blood upon application. This leads to the concentration of coagulation factors and platelets in the bleeding site, thereby, stimulating the fibrin clot formation (Lewis, Atlee, Mannone, Lin, & Goppelt, 2015; Xi et al, 2018). MPH‐based HA products are profitable due to their easy application, no risk of viral transmission, and low cost.…”