Experimental evidence regarding the influence of surface charge on the bridging flocculation mechanism

Schmitt, A.; Fern�ndez-Barbero, A.; Cabrerizo-V�lchez, M.; Hidalgo-�lvarez, R.

doi:10.1007/bf01182433

Cited by 15 publications

(10 citation statements)

References 14 publications

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“…A number of groups have investigated the adsorption of proteins onto colloidal particles. Such studies have essentially been limited to investigations of single protein layer adsorption. ,,− ,− Schmitt et al reported that a monomolecular layer of BSA was adsorbed onto PS latex particles from solutions of pH ≈ 5, which is in agreement with the FITC-BSA multilayer data obtained in the present study for adsorption of BSA onto PS latices from water. Kamyshny and Magdassi studied the adsorption of native and hydrophobically modified IgGs on hydrophobic polystyrene latex beads.…”

Section: Discussionsupporting

confidence: 88%

Protein Multilayer Formation on Colloids through a Stepwise Self-Assembly Technique

1999

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Multilayer films of the proteins fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) and immunoglobulin G (IgG) were assembled on 640 nm diameter polystyrene (PS) latex particles by their alternate deposition with either positively or negatively charged polyelectrolytes. The proteins and polyelectrolytes were deposited under conditions where they were oppositely charged to one another, thereby facilitating growth of the films through electrostatic interactions. The regular, controlled, stepwise assembly of the protein and polyelectrolyte multilayer films was followed by electrophoretic mobility (EPM), single particle light scattering (SPLS), and transmission electron microscopy (TEM) methods. EPM measurements reveal alternating negative and positive ζ-potentials with deposition of each protein and polyelectrolyte layer, respectively, for the FITC-BSA multilayer films. In contrast, no reversal of the surface charge occurred for the deposition of IgG and poly(sodium 4-styrenesulfonate) (PSS) in the construction of IgG multilayers, although an oscillatory behavior in ζ-potential was observed under the conditions studied. Both SPLS and TEM demonstrate that regular, stepwise protein multilayer growth occurs for both FITC-BSA and IgG. TEM also provides direct visual observation that the PS colloids are uniformly coated with protein. The stepwise, alternate assembly technique employed permits nanometer-level control over the thickness of the protein-polyelectrolyte shell surrounding the colloids. The formation of these novel, biologically functional, core-shell particles is expected to impact the areas of biotechnology and biochemical engineering.

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Section: Discussionsupporting

confidence: 88%

Protein Multilayer Formation on Colloids through a Stepwise Self-Assembly Technique

1999

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“…The use of nano‐ or sub‐micrometre particles with their extremely high surface‐to‐volume ratio in the immobilization of biomolecules dates back to the early 1980s 115. Both polymers116–119 and inorganic nanoparticles such as gold and silver120–126 have been successfully conjugated with proteins/enzymes. The choice of polymeric particles as biomolecule carriers has given rise to a great amount of research on the adsorption/grafting of protein molecules onto such dispersed material.…”

Section: Synthesis Characterization and Applications Of Composite MImentioning

confidence: 99%

Composite aqueous microgels: an overview of recent advances in synthesis, characterization and application

Pich

Adler

2006

Polymer International

104

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In the last decade there has been a steady increase in the number of publications describing the synthesis, characterization and applications of hybrid particles. Colloidal aqueous microgels form an important subdivision of polymer colloids used for the deposition of different functional materials. Due to their numerous attractive properties, microgel particles have been used as templates for the synthesis, storage and transportation of nanostructured materials. This paper reviews the important developments in the area of composite microgels over the last decade, and is limited to microgel particles operating in aqueous media. The patent literature is not included in the review. Different synthetic approaches as well as some properties and applications of composite microgels are discussed.

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“…A number of groups have studied the adsorption of proteins on both polymer (Basinska et al, 1999;Caruso et al, 1999;Elgersma et al, 1990;Molina-Bolivar and OrtegaVinuesa, 1999;Schmitt et al, 1997) and inorganic colloidal particles of oxides/metals. Recently, functionalized g-Fe 2 O 3 magnetic nanoparticles (Dyal et al, 2003) have been used as a support for the enzyme lipase.…”

Section: Introductionmentioning

confidence: 99%

Immobilization and biocatalytic activity of fungal protease on gold nanoparticle‐loaded zeolite microspheres

Phadtare

Vinod

Mukhopadhyay

et al. 2004

Biotech & Bioengineering

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Gold nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. However, separation of the gold nanoparticle-enzyme bioconjugate material from the reaction medium is often difficult. In this study, we investigate the assembly of the gold nanoparticles on the surface of the amine-functionalized zeolite microspheres in the formation of zeolite-gold nanoparticle "core-shell" structures and, thereafter, the use of this structure in immobilization of fungal protease. The assembly of gold nanoparticles on the zeolite surface occurs through the amine groups present in 3-aminopropyltrimethoxysilane (3-APTS). The fungal proteases bound to the massive "core-shell" structures were easily separated from the reaction medium by mild centrifugation and exhibited excellent reuse characteristics. The biocatalytic activity of fungal protease in the bioconjugate was marginally enhanced relative to the free enzyme in solution. The bioconjugate material also showed significantly enhanced pH and temperature stability and a shift in the optimum temperature of operation.

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Experimental evidence regarding the influence of surface charge on the bridging flocculation mechanism

Cited by 15 publications

References 14 publications

Protein Multilayer Formation on Colloids through a Stepwise Self-Assembly Technique

Protein Multilayer Formation on Colloids through a Stepwise Self-Assembly Technique

Composite aqueous microgels: an overview of recent advances in synthesis, characterization and application

Immobilization and biocatalytic activity of fungal protease on gold nanoparticle‐loaded zeolite microspheres

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