Experimental Forelimb Allotransplantation in Canine Model

Hong, Sa Hyeok; Eun, Seok Chan

doi:10.1155/2016/1495710

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…Canine forelimbs closely resemble human upper extremities in anatomical structure and vessel size (Hong & Eun, 2016). However, the canine MHC complex is not as well defined as in its porcine, or NHP counterparts.…”

Section: Discussionmentioning

confidence: 99%

“…Large animal models of upper extremity transplantation have included canines, nonhuman primate (NHP), and swine species. Canine forelimbs closely resemble human upper extremities in anatomical structure and vessel size (Hong & Eun, 2016). However, the canine MHC complex is not as well defined as in its porcine, or NHP counterparts.…”

Section: Discussionmentioning

confidence: 99%

“…However, the canine MHC complex is not as well defined as in its porcine, or NHP counterparts. Canine immune markers, tissue typing reagents, and probes are scarce (Brandacher et al, 2012; Hong & Eun, 2016; Kuo et al, 2006). More importantly, the public's emotional aversion to the use of dogs in research, make other large animal models preferred.…”

Section: Discussionmentioning

confidence: 99%

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Orthotopic forelimb transplantation in a Yucatan minipig model: Anatomic and in vivo study

Figueroa,

Ordenana,

Rezaei

et al. 2024

Microsurgery

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IntroductionAbove elbow transplants represent 19% of the upper extremity transplants. Previous large‐animal models have been too distal or heterotopic, did not use immunosuppression and had short survival. We hypothesize that an orthotopic forelimb transplant model, under standard immunosuppression, is feasible and can be used to address questions on peri‐transplant ischemia reperfusion injury, and post‐transplantation vascular, immunologic, infectious, and functional outcomes.Materials and methodsFour forelimbs were used for anatomical studies. Four mock transplants were performed to establish technique/level of muscle/tendon repairs. Four donor and four recipient female Yucatan minipigs were utilized for in‐vivo transplants (endpoint 90‐days). Forelimbs were amputated at the midarm and preserved through ex vivo normothermic perfusion (EVNP) utilizing an RBC‐based perfusate. Hourly perfusate fluid‐dynamics, gases, electrolytes were recorded. Contractility during EVNLP was graded hourly using the Medical Research Council scale. EVNP termination criteria included systolic arterial pressure ≥115 mmHg, compartment pressure ≥30 mmHg (at EVNP endpoint), oxygen saturation reduction of 20%, and weight change ≥2%. Indocyanine green (ICG) angiography was performed after revascularization. Limb rejection was evaluated clinically (rash, edema, temperature), and histologically (BANFF classification) collecting per cause and protocol biopsies (POD 1, 7, 30, 60 and endpoint). Systemic infections were assessed by blood culture and tissue histology. CT scan was used to confirm bone bridging at endpoint.ResultsAnimals 2, 4 reached endpoint with grade 0‐I rejection. Limbs 1, 3 presented grade III rejection on days 6, 61. CsA troughs averaged 461 ± 189 ng/mL. EVNLP averaged 4.3 ± 0.52 h. Perfusate lactate, PO2, and pH were 5.6 ± 0.9 mmol/L, 557 ± 72 mmHg and 7.5 ± 0.1, respectively. Muscle contractions were 4 [1] during EVNLP. Transplants 2, 3, 4 showed bone bridging on CT.ConclusionWe present preliminary evidence supporting the feasibility of an orthotopic, mid‐humeral forelimb allotransplantation model under standard immunosuppression regimen. Further research should validate the immunological, infectious, and functional outcomes of this model.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Orthotopic forelimb transplantation in a Yucatan minipig model: Anatomic and in vivo study

Figueroa,

Ordenana,

Rezaei

et al. 2024

Microsurgery

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show abstract

“…Although small animal models, including mice and rat, incur lower costs to acquire and maintain, there are several limitations of using small animals in transplantation research [1011]. Canines have strong similarities in surgical anatomy, physiology, and surgical techniques with humans and have contributed to the development of transplantation medicine fields [1213].…”

Section: Introductionmentioning

confidence: 99%

The degree of major histocompatibility complex matching between purebred Maltese and mongrel dogs using microsatellite markers

2019

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Long-term maintenance of transplanted organs is one of the major factors that increases survival time of recipients. Although obtaining a major histocompatibility complex (MHC)-matched donor with the recipient is essential for successful organ transplantation, there have been limited reports on MHC matching between dogs. In this study, we analyzed the canine MHC matching rates using Maltese, one of the most popular purebred dogs, and mongrel dogs in Korea. Genomic DNA was extracted from blood leukocytes and DNA was amplified by polymerase chain reaction with primers specific to MHC microsatellite markers. The MHC matching degree was confirmed by the microsatellite markers using polyacrylamide gel electrophoresis. The MHC matching rates of each donor-recipient groups including Maltese-Maltese, mongrel-mongrel and Maltese-mongrel were 4.76%, 5.13% and 6.67%, respectively. There were no significant differences in the MHC matching degree between each group. These results demonstrate that MHC-matched donors could be selected from other breeds as much as from the same breed for transplantation. Knowledge of the MHC matching degree of purebred and mongrel dogs would offer valuable information not only for improving the success rate of organ transplantation surgery in canine patients but also for transplantation research using experimental canine models.

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