Experimental glimpses at the lower trypanosomatidae

SYNOPSIS. Leishmania tareiatolar grown in Trager's defined medium grew best a t 21" and 28°C. There was a long lag phase at 33" with low population peaks. At 33" the long lag phase after each transfer showed that L. tarentolae had not completely adapted to the higher temperature. Red blood cell extract stimulated growth at 28" and permitted good growth at 33", indicating that the defined medium was not optimal. Sub-EASUREMENT of the effect of temperature M upon the growth, morphogenesis, and nutritional

Section: Jaroslav Weisersupporting

confidence: 80%

mentioning

confidence: 99%

Effect of Temperature on Growth and Nutritional Requirements of Leishmania tarentolae in a Defined Medium*

Krassner

1965

“…The growth of "L. pessoai" at 37 C in a defined medium, similar to that developed for other lower trypanosomatids, is unprecedented and inderd surprising in the light of previous attempts to grow various Trypanosomatidae at high temperatures (6, 8,15,22). The ability of the herpctomonad to grow in a carbohydrate-free medium ( Table 2 ) may be due to its ability to utilize amino acids, glyrerophosphate, or Krebs cycle intermediates as substrates.…”

Section: Discussionmentioning

confidence: 78%

Growth of an Insect Trypanosomatid at 37 C in a Defined Medium*

Roitman

Azevedo

1972

130

SYNOPSIS. A defined medium for an insect trypanosomatid, “Leptomonas pessoai” (probably a member of the genus Herpetomonas) isolated from the reduviid Zelus leucogrammus, allows growth up to 37 C. No marked differences were evident for growth at 37 C. The requirements for amino acids, vitamins, purine, and hemin, and pH range were like those established for Crithidia fasciculata. Again like C. fasciculata, it remains alive at 4 C for at least 3 months in a çlycerolated defined medium.

“…6). This corresponds roughly to the limit of whole-cell temperature tolerance in supplemented media (28). Above 35 C, O2 uptake decreased rapidly; at 50 C it was less than 20% of maximum.…”

Section: Supernatant (('Cytoplasmic") Enzymesmentioning

confidence: 73%

O₂‐Polarographic Studies on Soluble and Mitochondrial Enzymes of Crithidia fasciculata; Glycerophosphate Enzymes*

Bacchi¹,

Hutner²,

Ciaccio³

et al. 1968

SYNOPSIS. Mitochondrial and supernatant fractions were isolated from Crithidia fasciculata by grinding with neutral alumina and differential centrifugation. Supernatant fractions contained at least 2 NAD‐linked enzymes: an α‐glycerophosphate dehydrogenase and a malate dehydrogenase. The properties of these enzymes were investigated polarographically with phenazine ethosulfate acting as electron acceptor. Agaricic acid, cinnamic acid and p‐NO2‐cinnamic acid were specific inhibitors of the α‐glycerophosphate dehydrogenase. Succinate, malate, DL‐α‐glycerophosphate and NADH stimulated respiration of mitochondrial preparations; O2 uptake was greatest with succinate. KCN and antimycin A inhibited succinate respiration more than α‐glycerophosphate respiration. Amytal did not affect succinate, α‐glycerophosphate or NADH oxidation. The trypanocide suramin inhibited mitochondrial respiration at least 77% with each substrate. The relevance of these results to other members of the Trypanosomatidae is discussed.