Experimental human placental models for studying uptake, transport and toxicity of micro- and nanoplastics

Dusza, Hanna M.; Boxel, Jeske van; Duursen, Majorie B.M. van; Forsberg, Markus; Legler, Juliette; Vähäkangas, Kirsi

doi:10.1016/j.scitotenv.2022.160403

Cited by 32 publications

(4 citation statements)

References 184 publications

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“…Amereh et al (2022) delineated a potential dose-response relationship in humans between placental plastics and reduced fetal growth, suggesting possible interference in nutrient exchange in the placenta. This is supported by several in vitro studies showing that microplastic exposure is toxic for a variety of human placental cell lines (Lee et al, 2021;Dusza et al, 2022;Shen et al, 2022;Ragusa et al, 2022a;Dusza et al, 2023). In pregnant mice exposed to polystyrene microparticles, similar outcomes were found, demonstrating disruptions in placental metabolism Aghaei et al, 2022a;Aghaei et al, 2022b).…”

Section: Placental and Fetal Developmentsupporting

confidence: 59%

Microplastics: A threat for developing and repairing organs?

Hofstede,

Vasse,

Melgert

2023

Camb. prisms Plast.

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Plastic production has greatly increased in the past decades and has become central to modern human life. Realization is dawning that plastics break down into smaller pieces resulting in micro- or nanoplastics (MNP) that can enter humans directly via the environment. Indeed, MNP have been detected in every part of the human body, including the placenta, which is concerning for development. Early developmental stages are crucial for proper growth and genome programming. Environmental disruptors in MNP can have detrimental effects during this critical window as well and can increase the risk of developing disease and dysfunction. In addition, MNP may impact situations in which developmental pathways are reactivated after birth such as during organ repair. Currently, there is no overview of how MNP can impair (human) development and repair. Therefore, we provide an extensive overview of available evidence on MNP impacting developmental and regenerative processes in various organs in humans and rodent models. In addition, we have included the impact of some additives that can leach from these MNP. We conclude that MNP and their additives can have modulating effects on developing and regenerating organs.

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Section: Placental and Fetal Developmentsupporting

confidence: 59%

Microplastics: A threat for developing and repairing organs?

Hofstede,

Vasse,

Melgert

2023

Camb. prisms Plast.

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“…Mounting evidence suggest that MNPs accumulate within and affect the proper functioning of the placenta – the vital organ of pregnancy responsible for all maternal-fetal exchange ( 95 ).The presence of MNP accumulation in placental tissue of rats treated with PS-MNPs was first described in 2020 ( 96 ) and has since been reproduced in many studies in mice ( 97 – 99 ), with observed structural and functional consequences ( 96 – 101 ). Exposed females (MNPs between 100 nm-10 μm) have smaller placentas ( 96 , 98 ), reduced numbers of glycogen-containing cells within the placental endocrine-functioning junctional zone ( 100 ), and poorly developed feto-placental vasculature ( 100 ).…”

Section: Mnp Exposure In Pregnancymentioning

confidence: 99%

Microplastics exposure: implications for human fertility, pregnancy and child health

Zurub,

Cariaco,

Wade

et al. 2024

Front. Endocrinol.

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Plastics found in our everyday environment are becoming an increasing concern for individual and population-level health, and the extent of exposure and potential toxic effects of these contaminants on numerous human organ systems are becoming clear. Microplastics (MPs), tiny plastic particles, appear to have many of the same biological effects as their plastic precursors and have the compounded effect of potential accumulation in different organs. Recently, microplastic accumulation was observed in the human placenta, raising important questions related to the biological effects of these contaminants on the health of pregnancies and offspring. These concerns are particularly heightened considering the developmental origins of health and disease (DOHaD) framework, which postulates that in utero exposure can programme the lifelong health of the offspring. The current review examines the state of knowledge on this topic and highlights important avenues for future investigation.

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“…The STB layer undergoes continuous remodeling through the proliferation and differentiation of CTB cells into STB cells. The villi stroma is formed by connective tissue containing stromal cells, such as fibroblasts, fetal macrophages (Hoffbauer cells), and fetal vessels [3]. The cell layers that separate maternal blood from the fetal vessels are referred to as the placental barrier.…”

Section: Introductionmentioning

confidence: 99%

“…The cell layers that separate maternal blood from the fetal vessels are referred to as the placental barrier. In humans, the placental barriers placental barriers include the STB, CTB, trophoblast basal lamina, cellular and extracellular components of connective tissue, and the endothelium of the fetal capillaries [3].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Integrity and Function of Human Term Placental Explants in Short-Term Culture

López-Guzmán,

García,

Marín

et al. 2024

MPs

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Human placental explants (HPEs) culture has generated significant interest as a valuable in vitro model for studying tissue functions in response to adverse conditions, such as fluctuations in oxygen levels, nutrient availability, exposure to pathogenic microorganisms, and toxic compounds. HPEs offers the advantage of replicating the intricate microenvironment and cell-to-cell communication involved in this critical and transient organ. Although HPEs culture conditions have been extensively discussed, a protocol for assessing the viability and function of HPEs during short-term culture has not been previously outlined. In this study, we have developed a short-term HPEs culture protocol, specifically up to 72 h, and have employed quantitative, semi-quantitative, and qualitative analyses to evaluate tissue viability and function over time. Under our standardized conditions, placental villi explants began to regain their structural properties (the integrity of the trophoblast and villous stroma) and the functionality of the HPEs (production of angiogenic, endocrine, and immunological factors) starting from 48 h of culture. This restoration ensures a suitable environment for several applications. The data presented here can be highly valuable for laboratories aiming to implement an HPEs model, whether in the process of standardization or seeking to enhance and optimize working conditions and timing with placental tissue.

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Experimental human placental models for studying uptake, transport and toxicity of micro- and nanoplastics

Cited by 32 publications

References 184 publications

Microplastics: A threat for developing and repairing organs?

Microplastics: A threat for developing and repairing organs?

Microplastics exposure: implications for human fertility, pregnancy and child health

Assessment of the Integrity and Function of Human Term Placental Explants in Short-Term Culture

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