Experimental hybridization in abalones

Leighton, D. L.; Lewis, Cindy

doi:10.1080/01651269.1982.10553479

Cited by 57 publications

(59 citation statements)

References 1 publication

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“…In vitro hybridization experiments have shown that optimal sperm concentrations are ten times higher and maximal fertilization rates much lower for heterospecific than for conspecific sperm (Leighton and Lewis, 1982). Moreover, dissolution of the VE by purified lysin and, to a lesser extent, in vitro binding of lysin and VERL, show the same species specificity as does egg-sperm binding (Swanson and Vacquier, 1997;Lyon and Vacquier, 1999), demonstrating that the interaction of these two proteins is the species-specific step of fertilization in abalones.…”

Section: Gamete Interaction In Abalonesmentioning

confidence: 69%

Causes and consequences of the evolution of reproductive proteins

Turner

Hoekstra²

2008

Int. J. Dev. Biol.

110

154

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Proteins involved in reproduction often evolve rapidly, raising the possibility that changes in these proteins contribute to reproductive isolation between species. We review the evidence for rapid and adaptive change in reproductive proteins in animals, focusing on studies in recently diverged vertebrates. We identify common patterns and point out promising directions for future research. In particular, we highlight the ways that integrating the different but complementary approaches of evolutionary and developmental biology will provide new insights into fertilization processes.

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Section: Gamete Interaction In Abalonesmentioning

confidence: 69%

Causes and consequences of the evolution of reproductive proteins

Turner

Hoekstra²

2008

Int. J. Dev. Biol.

110

154

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“…Leighton & Lewis (1982) also found that at sperm concentrations >10 8 sperm ml -1 , lysis of the vitelline layer occurred. Clavier (1992) found a variable optimum sperm concentration when examining fertilization success in H. tuberculata in 10 ml culture volume.…”

Section: Discussionmentioning

confidence: 92%

“…This is a similar range to that found for H. tuberculata. In the Californian coastal species H. rufescens, H. corrugata, H. fulgens and H. sorenseni, Leighton & Lewis (1982) demonstrated that the concentration of spermatozoa that yielded maximum fertilization with least abnormality in subsequent development was ca 10 6 sperm ml -1 . The production of hybrid zygotes from these 4 species required an optimum sperm concentration of 10 7 sperm ml -1…”

Section: Discussionmentioning

confidence: 99%

Fertilization success in the commercial gastropod Haliotis tuberculata

Baker¹,

Tyler²

2001

Mar. Ecol. Prog. Ser.

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A series of laboratory experiments was conducted to determine fertilization success in the commercial abalone Haliotis tuberculata L. Fertilization success is a function of sperm dilution, sperm-egg ratio, gamete age and the amount of time sperm and eggs are in contact. Sperm density required for optimum fertilization success in vivo, using 10 ml culture volume, was in the range 5 × 10 4 to 5 × 10 5 sperm ml -1. When these experiments were repeated using 500 ml culture volume, results showed high levels of fertilization success across a far larger range of sperm dilutions with success > 80% at 10 3 sperm ml -1. Sperm-egg ratios of >100:1 have little effect on fertilization success, but below these levels this factor becomes significant. Viability of both sperm and eggs decreased with age so that no fertilization occurred after 2.5 h. Maximum fertilization success occurred after 30 min sperm-egg contact time. These data suggest that sperm limitation may severely compromise reproductive success in exploited populations of H. tuberculata.

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“…Surface proteins involved in sperm-egg interactions are better characterized for abalone (genus Haliotis) than for any other taxon, and demonstrate strong selection for species-specific gamete recognition (5). There are Ϸ60 species of abalone worldwide, many with overlapping breeding seasons and habitats, yet hybrids are rare (27,28). It is unresolved whether remote chemical communication between sperm and eggs is important in maintaining reproductive isolation among extant abalone species that could potentially hybridize.…”

Section: Discussionmentioning

confidence: 99%

“…Amid shallow-water rocky reefs, male and female abalone broadcast gametes into the ocean, and thus, fertilization occurs externally to the body cavity. Within this turbulent fluid environment, spawned eggs and sperm are rapidly diluted below critical densities for successful fertilization (28). Sperm may be under intense selective pressure to recognize eggs at a distance, due to competition for limited egg resources, or because gamete dilution quickly diminishes mating opportunities.…”

Section: Discussionmentioning

confidence: 99%

The ecological and evolutionary consequences of sperm chemoattraction

Riffell

Krug

Zimmer

2004

Proc. Natl. Acad. Sci. U.S.A.

104

112

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Chemical communication between sperm and egg is a critical factor mediating sexual reproduction. Sperm attractants may be significant evolutionarily for maintaining species barriers, and important ecologically for increasing gamete encounters. Still unresolved, however, are the functional consequences of these dissolved signal molecules. Here, we provide experimental evidence that sperm chemoattraction directly affects the magnitude of fertilization success. The recent discovery of L-tryptophan as a potent attractant to red abalone (Haliotis rufescens) sperm affords the opportunity to quantify how navigation affects gamete interactions. Sperm behavioral responses to manipulations of the natural tryptophan gradient around individual eggs reveals that both chemotaxis and chemokinesis significantly promote contacts. Our results show further that attractant release by means of diffusion effectively doubles the target size of red abalone eggs, which in turn significantly increases fertilization success. Although long theorized as potential barriers to hybridization, species-specific sperm attractants in red and green (Haliotis fulgens) abalone are only minor contributors to maintaining reproductive isolation. Because abalone typically live in dense, multispecies aggregations, chemically mediated navigation would prevent sperm from pointlessly tracking heterospecific eggs. Thus, even though reproductive isolation fundamentally resides at the level of membrane recognition proteins, species-specific sperm attractants may evolve to locate the right target within mixed gamete suspensions of closely related species.

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Experimental hybridization in abalones

Cited by 57 publications

References 1 publication

Causes and consequences of the evolution of reproductive proteins

Causes and consequences of the evolution of reproductive proteins

Fertilization success in the commercial gastropod Haliotis tuberculata

The ecological and evolutionary consequences of sperm chemoattraction

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