Experimental <i>Nosema ceranae</i> infection is associated with microbiome changes in the midguts of four species of <i>Apis</i> (honey bees)

Naree, Sanchai; Benbow, M. Eric; Suwannapong, Guntima

doi:10.1080/00218839.2021.1987086

Cited by 4 publications

(4 citation statements)

References 58 publications

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“…This positive association between G. apicola and experimental N. ceranae infection seems to be very common, as it was found by other authors [56,58,[62][63][64] in trials conducted under very different conditions, addressing infection at different ages, different honey bee subspecies, distinct spore doses, gut regions examined, diets fed, analytical methods, or through the analysis of individual bees or pooled samples. However, others failed to find any association between N. ceranae infection and this bacterium or with any other bacteria [65], while other studies found associations with other bacteria like those of the genera Lactobacillus, Bifidobacterium, Snodgrassella, or Bartonella [61,62,[66][67][68][69], which did not appear to be modified here. Most of these studies used very young bees (24-48 h p.e.)…”

Section: Discussioncontrasting

confidence: 53%

Influence of Age of Infection on the Gut Microbiota in Worker Honey Bees (Apis mellifera iberiensis) Experimentally Infected with Nosema ceranae

Aguado-López,

Urbieta Magro,

Higes

et al. 2024

Microorganisms

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The gut microbiota of honey bees has received increasing interest in the past decades due to its crucial role in their health, and can be disrupted by pathogen infection. Nosema ceranae is an intracellular parasite that affects the epithelial cells of the midgut, altering gut homeostasis and representing a major threat to honey bees. Previous studies indicated that younger worker bees are more susceptible to experimental infection by this parasite, although the impact of infection and of age on the gut bacterial communities remains unclear. To address this, honey bees were experimentally infected with a consistent number of N. ceranae spores at various ages post-emergence (p.e.) and the gut bacteria 7 days post-infection (p.i.) were analysed using real-time quantitative PCR, with the results compared to non-infected controls. Infected bees had a significantly higher proportion and load of Gilliamella apicola. In respect to the age of infection, the bees infected just after emergence had elevated loads of G. apicola, Bifidobacterium asteroides, Bombilactobacillus spp., Lactobacillus spp., Bartonella apis, and Bombella apis. Moreover, the G. apicola load was higher in bees infected at nearly all ages, whereas older non-infected bees had higher loads of Bifidobacterium asteroides, Bombilactobacillus spp., Lactobacillus spp., Ba. apis, and Bo apis. These findings suggest that N. ceranae infection and, in particular, the age of bees at infection modulate the gut bacterial community, with G. apicola being the most severely affected species.

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Section: Discussioncontrasting

confidence: 53%

Influence of Age of Infection on the Gut Microbiota in Worker Honey Bees (Apis mellifera iberiensis) Experimentally Infected with Nosema ceranae

Aguado-López,

Urbieta Magro,

Higes

et al. 2024

Microorganisms

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“…A decline in the numbers of Bifidobacterium spp. is also observed in insects infected with Nosema ceranae (Zhang et al, 2019;Naree et al, 2022). Cuesta-Maté et al (2021) report that oxalic acid used in Varroa destructor infected apiaries also exhibits bactericidal activity against Lactobacillus strains isolated from the intestines of the insects.…”

Section: Discussionmentioning

confidence: 88%

Intestinal Microbiota of Honey Bees (Apis mellifera) Treated with Amitraz

Żebracka

Chmielowiec-Korzeniowska

Nowakowicz-Dębek

et al. 2022

Journal of Apicultural Science

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The aim of the study was to analyse the intestinal microbiota of honey bees (Apis mellifera) treated with amitraz. In the present study, the microbiological profile of the honey bee intestines showed minor changes in the microbiota following the application of amitraz. A comparison of the numbers of bacteria and fungi revealed a positive downward trend in the number of fungi. The number of decreased bacteria, included E. coli and especially Clostridium spp., which were not isolated from the intestines of bees treated with amitraz. The number of lactic acid bacteria before and after treatment was at a similar level of 8.3 log cfu/g of intestinal contents.

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“…However, infected bees treated with stingless bee propolis and COS had significantly reduced infection loads and midgut cells, resulting in more prolonged survival than the infected bees that did not receive any treatment. This is important to note because N. ceranae has been documented to infect both native and imported species of honey bees in Thailand [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…The tropical climate in Thailand could be essential in enhancing the infection of N. ceranae among Apis species. It seems clear that N. ceranae has become distributed throughout Thailand [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Chito-Oligosaccharide and Propolis Extract of Stingless Bees Reduce the Infection Load of Nosema ceranae in Apis dorsata (Hymenoptera: Apidae)

Ponkit

Naree

Pichayangkura

et al. 2022

JoF

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Nosema ceranae is a microsporidian that infects Apis species. Recently, natural compounds have been proposed to control nosemosis and reduce its transmission among honey bees. We investigated how ethanolic extract of Tetrigona apicalis’s propolis and chito-oligosaccharide (COS) impact the health of N. ceranae-infected Apis dorsata workers. Nosema ceranae spores were extracted from the guts of A. florea workers and fed 106 spores dissolved in 2 µL 50% (w/v) sucrose solution to A. dorsata individually. These bees were then fed a treatment consisting either of 0% or 50% propolis extracts or 0 ppm to 0.5 ppm COS. We found that propolis and COS significantly increased the number of surviving bees and lowered the infection ratio and spore loads of N. ceranae-infected bees 14 days post-infection. Our results suggest that propolis extract and COS could be possible alternative treatments to reduce N. ceranae infection in A. dorsata. Moreover, N. ceranae isolated from A. florea can damage the ventricular cells of A. dorsata, thereby lowering its survival. Our findings highlight the importance of considering N. ceranae infections and using alternative treatments at the community level where other honey bee species can act as a reservoir and readily transmit the pathogen among the honey bee species.

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Experimental Nosema ceranae infection is associated with microbiome changes in the midguts of four species of Apis (honey bees)

Cited by 4 publications

References 58 publications

Influence of Age of Infection on the Gut Microbiota in Worker Honey Bees (Apis mellifera iberiensis) Experimentally Infected with Nosema ceranae

Influence of Age of Infection on the Gut Microbiota in Worker Honey Bees (Apis mellifera iberiensis) Experimentally Infected with Nosema ceranae

Intestinal Microbiota of Honey Bees (Apis mellifera) Treated with Amitraz

Chito-Oligosaccharide and Propolis Extract of Stingless Bees Reduce the Infection Load of Nosema ceranae in Apis dorsata (Hymenoptera: Apidae)

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