Experimental in vitro endothelialization of cardiac valve leaflets

Eberl, Thomas; Siedler, Susanne; Schumacher, B.; Zilla, Peter; Schlaudraff, K.; Fasol, Roland

doi:10.1016/0003-4975(92)90275-9

Cited by 36 publications

(12 citation statements)

References 16 publications

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“…At the height of in-vitro endothelialisation of vascular grafts, the concept was also applied to bioprosthetic heart valves. Glutaraldehyde detoxification made it possible to grow endothelial cells to confluence on bioprosthetic leaflets (219)(220)(221)(222)(223). Such in-vitro lined tissue valves maintained endothelial integrity in the heterotopic primate model (224,225) and even showed some degree of mitigation of tissue calcification (225, 226).…”

Section: Two-stage Co-culture Of Scaffolds and Cellsmentioning

confidence: 99%

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

Zilla

Deutsch

Bezuidenhout

et al. 2020

Front. Cardiovasc. Med.

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Section: Two-stage Co-culture Of Scaffolds and Cellsmentioning

confidence: 99%

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

Zilla

Deutsch

Bezuidenhout

et al. 2020

Front. Cardiovasc. Med.

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“…Endothelial cell seeding is an effective method of preventing thromboembolism on surfaces of cardiovascular implants and biodevices because endothelial cells release chemical agents and proteins that block platelet adhesion and fibrin formation 1–3. Seeding of vascular graft prosthesis with endothelial cells before implantation significantly increases graft patency and survival 4, 5.…”

Section: Introductionmentioning

confidence: 99%

Differential regulation of endothelial cell adhesion, spreading, and cytoskeleton on low‐density polyethylene by nanotopography and surface chemistry modification induced by argon plasma treatment

Tajima

Chu

et al. 2007

J Biomedical Materials Res

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Plasma treatment of polymer surfaces can modify the nanoscale roughness, wettability, and oxygen surface functionalities. However, how these modifications regulate cell behavior is not well understood. The objective of this investigation was to examine adhesion, spreading, and cytoskeleton of vascular endothelial cells seeded on low-density polyethylene surfaces modified by Ar plasma. In the absence of serum, adhesion and spreading of the cells and actin filament assembly were enhanced by high-energy Ar plasma-induced hydrophilicity and formation of C-O groups at the surface. Although serum increased cell adhesion and spreading on untreated surfaces for a relatively short period, this behavior was not stable for a long time. In contrast to the untreated polymer surfaces, serum suppressed cell adhesion and spreading on the plasma-treated surfaces. The preadsorption of albumin from the bovine serum on the polymer surfaces inhibited cell adhesion and spreading. Results demonstrate the differential effects of Ar plasma-induced surface modifications on endothelial cell behavior and provide insight into complex interactions among polymer surfaces, adsorbed proteins, and cells. The findings of this study have significant implications in surface engineering for vascular repair.

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“…Our own comparative study of endothelial cell proliferation and morphology on differently treated valve surfaces clearly favored valves either nonpreserved, like commonly used homograft valves, or preserved due to a mild, alternative fixation technique. 262 In summary, the creation of a viable, autologous endothelial lining on the surface of biological heart valves, either by in vitro lining, by microvascular seeding, or by growth factor mediated facilitated ingrowth, promises to establish the most physiological of all barriers between tissue and the blood stream. lt would not be surprising if such a tissue protection resulted in a dramatic reduction of bioprosthetic degeneration with a concomitant revival of the concept of biological heart valves on a broad basis.…”

Section: Endothellallzation Of Artlflclal Heartsmentioning

confidence: 99%

The Endothelium: A Key to the Future

1993

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The vascular endothelium is a complex modulator of a variety of biological systems and may well be the key to definitive success in the treatment of cardiovascular disorders. Surgically-induced endothelial injury may occur preoperatively during cardiac catheterization and intraoperatively from mechanical manipulation, ischemia, hypothermia, and exposure to cardioplegic solutions. The normal endothelium is antithrombogenic and yet promotes platelet aggregation and coagulation if injured. Vasospasm, occlusive intimal hyperplasia, and accelerated arteriosclerosis can also all occur as a result of endothelial injury. Furthermore, endothelial injury is harmful even in the absence of disruption of its monolayer integrity. Thus, preservation of the endothelium should be an additional objective for all cardiovascular surgeons. Synthetic vascular grafts, cardiac valves, and artificial ventricles do not spontaneously endothelialize and thus usually require some form of anticoagulation to maintain patency. Hence, endothelialization of prosthetic implants became an attractive concept. A number of different methods of obtaining an endothelial lining of prosthetic material has since been developed; these include facilitated endothelial cell migration, and endothelial cell seeding by using either venous or microvascular endothelial cells. Manipulating the endothelium might well provide the next major advancement for therapeutic and preventative measures for cardiovascular disease. (J Card Surg 1993;8:32-60)In 1628 William Harvey discovered the circulation of the blood and yet, even in the 1950s with the advent of vascular prostheses and cardiopulmonary bypass, the vascular endothelium was still considered to be merely a hemocompatible barrier to the blood stream. However, this monolayer of squamous cells (Fig. l), which has a total surface area of approximately 1,000 m 2,1,2 is now known to be a complex modulator of a variety of biological systems.In spite of this deepened understanding of endothelial cell function, the causative association of endothelial injury and surgical failure is still not appropriately appreciated. The reason for this negligence of cell biological events lies in the endurance of a mechanistic attitude, typical for the early days of surgery. Part of this attitude is the continual conviction of many surgeons that most of the severe cardiovascular disorders are mechanical in nature and thus susceptible to mechanical solutions. The astonishing success of surgical techniques further explains why the vast majority of surgeons realized only so late that the limitations of their techniques were of a biological rather than mechanical nature. Meanwhile, physiologists and cell biologists have long identified the endothelium as the key to definite success in the treatment of cardiovascular disorders.As an initial step toward the integration of surgeons into current scientific concepts of therapy, a modern cardiovascular surgeon needs to understand the basic function of endothelium, its extent of intraoper...

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Experimental in vitro endothelialization of cardiac valve leaflets

Cited by 36 publications

References 16 publications

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

Progressive Reinvention or Destination Lost? Half a Century of Cardiovascular Tissue Engineering

Differential regulation of endothelial cell adhesion, spreading, and cytoskeleton on low‐density polyethylene by nanotopography and surface chemistry modification induced by argon plasma treatment

The Endothelium: A Key to the Future

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