Experimental Infection of Enterotoxemic Escherichia coli Associated with Porcine Edema Disease and its Pathologic Characteristics in the Intestine

et al. 2008

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ABSTRACT. The concentration of fecal secretory immunoglobulin A (sIgA) in neonate and weaning piglets was measured daily from 1 day after birth to 50 days of age. The concentration of fecal sIgA started from the level of 10 4 µg/g wet feces 1 day after birth and then increased to a maximal value of up to 10 5 µg/g within a few days of birth. The values constantly declined to between 10 1 and 10 2 µg/ g for the next 10 days and were relatively constant until weaning. The level of sIgA in the feces remained very low until at least 50 days of age. The vulnerability of pre-or post-weaning piglets can be explained, at least in part, by this low level of sIgA in the intestine. KEY WORDS: feces, immunoglobulin A, piglet.

mentioning

confidence: 99%

Decreasing Traits of Fecal Immunoglobulin A in Neonatal and Weaning Piglets

Ushida

Kameue

et al. 2008

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“…Clinical signs of diarrheal feces were checked daily using the following score (0, normal feces; 1, loose stool; 2, moderate diarrhea; 3, severe diarrhea) as described previously [14]. Body weight was measured on d0, d3 and d10.…”

Section: Methodsmentioning

confidence: 99%

Alpha-Linked Galactooligosaccharide Suppresses Small Intestinal Eosinophil Infiltration and Improves Growth Performance in Weaning Pigs

Inoüe

Shimojo

et al. 2010

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ABSTRACT. A healthy pig shows significant eosinophil infiltration in the lamina propria of the small intestine. Although the exact role of these infiltrated eosinophils in healthy pigs is unclear, eosinophil infiltration is a well-known phenomenon that is frequently observed during an allergic status. Alpha-linked galactooligosaccharide (GOS) reduces eosinophil infiltration into broncho-alveolar lavage fluid of an allergic airway eosinophilia model. We evaluated the effect of GOS oral administration on the suppression of eosinophil infiltration in the small intestine of healthy weaned pigs. Nine 21-day-old pigs were purchased and divided into three groups. One group was fed the basal diet supplemented with sucrose at 0.11% (C), one group was fed the basal diet supplemented with GOS at 1.17% (GOS A) and one group was fed the basal diet supplemented with GOS at 0.03% (GOS B). Each group was fed its respective diet throughout this study (10 days). The daily body weight gain from d3 to d10 was significantly bigger in the GOS B group than in the other groups. The feed conversion ratios from d0 to d10 were two times lower in the GOS B group than in the C group. Dietary GOS suppressed eosinophil infiltration in the small intestine. However, GOS administration had no effect on the organic acid level or microbial composition in the small and large intestinal digesta.

“…The animals were healthy and did not receive any antimicrobials prior to the study. They were checked to be negative for infection with STEC by the method as described previously [19]. The pigs were housed in pens at the KYODOKEN Institute, Kyoto, Japan.…”

Section: Animals and Basal Dietsmentioning

confidence: 99%

“…Source and nature of STEC: E. coli strain 1362-1 sero group O139, obtained from Dr. M. Sueyoshi, Miyazaki University, Miyazaki, Japan, was used to induce ED in pigs in the same manner as described previously [19]. Strain 1362-1 is hemolytic and the producer of Shiga toxin 2e, a heatstable enterotoxin, and the F18 fimbriae, a mediator of colonization on the intestine of weaning pigs [3].…”

Section: Animals and Basal Dietsmentioning

confidence: 99%

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Evaluation of the Low Dose Level of a Heat-Killed and Dried Cell Preparation of Enterococcus faecalis to Prevent Porcine Edema Disease Using Experimental Infection Model with Enterotoxcemic Escherichia coli in Weaning Pigs

Inoüe

Nakanishi³

et al. 2007

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ABSTRACT. Porcine edema disease (ED) is caused by Shiga toxin 2e-producing Escherichia coli (STEC). ED has become frequent in pig farms, and the use of antimicrobials has resulted in the development of antimicrobial-resistant STEC. Accordingly, the use of materials other than antimicrobials is requested for the prevention of ED. Oral administration of a heat-killed and dried cell preparation of Enterococcus faecalis strain EC-12 (EC-12) to weaning pigs was previously demonstrated to decrease animal mortality in a STEC-contaminated farm at 0.05% (w/w) dose level. In this study, pigs experimentally infected with STEC were used as a model for ED to evaluate the low dose level of EC-12 to prevent ED. Fifteen 21-day-old pigs were divided into 5 groups: STEC challenge with the basal diet, STEC challenge with EC-12 supplemented at 0.005, 0.01, or 0.05% (w/w) to the basal diet, and no STEC challenge with the basal diet. The challenge was carried out when the animals were 25, 26, and 27 days old using STEC contained in capsules resistant against gastric digestion. All pigs were euthanized at 32 days of age. The daily weight gain, feed conversion ratio, and palpebral edema were improved by supplementation with 0.05% EC-12, but not by the low dose levels. Accordingly, 0.05% level of supplementation was needed for EC-12 to improve clinical symptoms in weaning piglets infected by STEC. KEY WORDS: Enterococcus faecalis strain EC-12, enterotoxcemic Escherichia coli, experimental infection, porcine edema disease.