2013
DOI: 10.3329/pa.v22i1-2.16472
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Experimental Infection of Indigenous Climbing Perch <i>Anabas testudineus</i> with <i>Aeromonas hydrophila</i> Bacteria

Abstract: The present study was conducted to know the pathogenicity and LD 50 of Aeromonas hydrophila isolated from diseased climbing perch Anabas testudineus against apparently healthy homologous fish and the distribution of the bacteria in the organs of the experimentally infected fish. A total of 10 fish of average body weight of 18 g were used. For pathogenicity test, two different doses viz. 9.2 × 10 7 and 9.2 × 10 6 CFU/fish were injected intramuscularly. Pathogenicity of A. hydrophila was confirmed at water tempe… Show more

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Cited by 16 publications
(12 citation statements)
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“…Based on the bacterial isolation from the fish after the challenge, the intestine showed higher bacteria load than liver. This was also observed in the climbing perch with 9.4×10 8 cfu g -1 and 2.9×10 6 cfu g -1 in intestine and liver respectively (Hossain et al 2013). In addition, Asian stinging catfish, Heteropneustes fossilis also exhibited higher levels of bacteria accumulation in intestine (1.8×10 9 cfu g -1 ) than liver (6.46×10 8 cfu g -1 ) (Mostafa et al 2008).…”
Section: Discussionmentioning
confidence: 82%
“…Based on the bacterial isolation from the fish after the challenge, the intestine showed higher bacteria load than liver. This was also observed in the climbing perch with 9.4×10 8 cfu g -1 and 2.9×10 6 cfu g -1 in intestine and liver respectively (Hossain et al 2013). In addition, Asian stinging catfish, Heteropneustes fossilis also exhibited higher levels of bacteria accumulation in intestine (1.8×10 9 cfu g -1 ) than liver (6.46×10 8 cfu g -1 ) (Mostafa et al 2008).…”
Section: Discussionmentioning
confidence: 82%
“…Skin samples from A. hydrophila infected blue tilapia from ponds of the Marine Center Science, Unversity of Basrah, were transferred to tryptic soy agar (TSA) medium and incubated, resulting in the growth of many bacterial colonies. A. hydrophila was identified according to (Hossain et al, 2013); macroscopic observations included size, shape and pigmentation seen from inside, beside and top; microscopic analysis was done by Gram staining and observing the color and shape of bacteria; biochemical tests were: Motile, indol, O/F and H 2 S. A specific medium, Rimler-Shotts Agar, was used to confirm the isolated bacteria (Sayed, 2010). Cell densities were photometrically obtained at a wavelength of 590 nm (Saulnier et al, 2000), based on the McFarland standard and serially diluted to reach the target density (1 × 10 7 CFU).…”
Section: Methodsmentioning
confidence: 99%
“…The intramuscular injection technique, a method applied by several investigators [18][19][20] was used to detect both the effectiveness of the method in initiating the disease and the pathogenicity of the bacteria. One ml of sterile and disposable insulin needles was used for intramuscular injection.…”
Section: Pathogenicity Experimentsmentioning
confidence: 99%