Experimental infection of Korean native goats (Capra aegagrus hircus) with bovine viral diarrhea virus 1b

Oem, Jae‐Ku; Han, Du-Gyeong; Choi, Kwang Shik

doi:10.1186/s12917-019-1955-0

Cited by 2 publications

(5 citation statements)

References 28 publications

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“…The consequences of BVDV infection in heterologous hosts vary depending on the host- and virus-associated factors such as biotype, genotype, or antigenic variation. However, they are generally similar to those in cattle [ 9 - 11 ]. The typical clinical symptom of BVDV infection in goats is reproductive failure [ 7 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 71%

“…However, they are generally similar to those in cattle [ 9 - 11 ]. The typical clinical symptom of BVDV infection in goats is reproductive failure [ 7 , 11 , 12 ]. In the previous studies, natural BVDV infection in healthy goats was rarely reported, and the goats rarely became reinfected after recovery from infection.…”

Section: Introductionmentioning

confidence: 71%

“…This finding is in agreement with previous reports [ 12 , 18 ]. BVDV infection (natural or experimental) in small ruminants during pregnancy can cause reproductive failures such as fetal resorption, fetal mummification, stillbirth, or abortion [ 11 ]. However, the clinical manifestations of BVDV infection during pregnancy in heterologous hosts are variable and depend on different host- and virus-associated factors but are generally characterized by reproductive disorders such as poor conception, intrauterine infection, abortion, and poor neonate viability [ 26 - 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, BVDV in goats was assumed not to be a problem and therefore did not require systematic control [ 13 - 15 ]. However, there is increasing serological evidence of Pestivirus infection in goats in many countries, including Indonesia [ 9 , 10 , 16 , 17 ], and BVDV-1a, BVDV-1b, and BVDV-2a have been reported in experimentally inoculated goats [ 9 , 11 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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First study on genetic variability of bovine viral diarrhea virus isolated from Sapera dairy goats with reproductive disorders in Yogyakarta, Indonesia

et al. 2022

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Background and Aim: Bovine viral diarrhea (BVD) virus (BVDV) is an important viral pathogen of cattle that can infect diverse artiodactyl species. The clinical manifestations caused by BVDV in heterologous hosts, as they do in cattle, vary, although respiratory and reproductive failures are commonly reported. BVDV infections commonly result in reproductive failure in goats, with abortion being the primary clinical sign. In central Java, Indonesia, BVDV infection has been reported in two clinically healthy local goat species, and the testing indicated infection by BVDV Type 1. However, the genetic diversity of viruses has not been described in healthy or ill goats. The objectives of the present study were as follows: (1) To investigate the genetic variation of BVDV isolated from Sapera dairy goats with naturally occurring reproductive disorders in Yogyakarta, Indonesia, using the 5' untranslated region (5' UTR) and (2) to study the possible correlation between reproductive disorders and the presence of BVDV in the flock. Materials and Methods: Blood samples were collected in October 2021 from 39 goats that had been individually reported to have reproductive disorders. The serum samples were subjected to molecular detection and genetic characterization of BVDV based on the 5' UTR of the viral genome, followed by sequencing and phylogenetic analyses. Viral isolation was performed on BVDV-positive samples to analyze the viral biotypes. Results: BVDV infection was detected in five out of 39 female goats. The clinical status of the BVDV-infected goats was abortion (n=2), metritis (n=1), and repeated breeding (n=2). All antigen-positive samples were confirmed as BVDV type 1a (BVDV-1a) and noncytopathic (NCP)-BVDV biotype. Conclusion: The BVDV-1a and NCP biotypes are the main subtypes and biotypes present in Sapera dairy goats exhibiting reproductive failure. This result is consistent with previous results in dairy cattle in Yogyakarta. The reported results can facilitate the design of methods for the prevention and control of BVD circulating in Indonesia.

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Section: Introductionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

First study on genetic variability of bovine viral diarrhea virus isolated from Sapera dairy goats with reproductive disorders in Yogyakarta, Indonesia

et al. 2022

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“…About 3 ml peripheral blood were collected from PARDS patients and healthy children. A total RNA blood kit (ThermoFisher, USA) was used to extract total RNA [ 20 ]. The concentration and purity of RNA have been detected by measuring their optical density (OD) 260 and OD 280 [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of microRNA-101-3p on predicting pediatric acute respiratory distress syndrome and its role in human alveolar epithelial cell

Yin

Min

et al. 2022

Bioengineered

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Pediatric acute respiratory distress syndrome (PARDS) is a severe form of respiratory failure associated with high mortality among children. The objective of this study is reported to explore the clinical function and molecular roles of microRNA-101-3p (miR-101-3p) in PARDS. The levels of miR-101-3p and mRNA levels of SRY-related high-mobility group box 9 (Sox9) were measured by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Additionally, the diagnostic role of miR-101-3p was identified by using the Receiver operating characteristic (ROC) curve. The cell proliferation and apoptosis were examined through Cell Counting Kit-8 (CCK-8) assay and flow cytometry. To detect inflammation in cells, enzyme-linked immunosorbent assays (ELISA) were performed. The target gene of miR-101-3p was confirmed through data obtained from the luciferase activity. In patients with PARDS, miR-101-3p expression was decreased. Moderate and severe PARDS patients had lower levels of miR-101-3p than mild PARDS patients. The inflammatory progression was related to the aberrant expression of miR-101-3p in all PARDS children. MiR-101-3p was highly predictive for the detection of children with PARDS. In addition, miR-101-3p might protect A549 cells from abnormal proliferation, apoptosis, and inflammation caused by lipopolysaccharide (LPS). Sox9 might be a target gene of miR-101-3p and increased mRNA expression of Sox9 in LPS-treated A549 cells was inhibited by overexpression of miR-101-3p. Ultimately, this study suggested that reduced expression of miR-101-3p plays a role in PARDS, providing a novel angle to study the disease.

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Experimental infection of Korean native goats (Capra aegagrus hircus) with bovine viral diarrhea virus 1b

Cited by 2 publications

References 28 publications

First study on genetic variability of bovine viral diarrhea virus isolated from Sapera dairy goats with reproductive disorders in Yogyakarta, Indonesia

First study on genetic variability of bovine viral diarrhea virus isolated from Sapera dairy goats with reproductive disorders in Yogyakarta, Indonesia

Effects of microRNA-101-3p on predicting pediatric acute respiratory distress syndrome and its role in human alveolar epithelial cell

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