This paper presents the results of experimental investigations on six-layered, homogeneous glulam beams made of Italian short supply chain beech (Fagus sylvatica L.). At first, the beams were produced and mechanically characterized for bending, then, they were employed to realize timber-timber composite joints and tested under quasi-static monotonic loading. The test configurations were adopted to reproduce connections used in timber-to-timber composite structures for applications in new constructions. Outcomes in terms of connection stiffness, strength, static ductility and failure modes are presented and discussed. Moreover, the experimental stiffness were used to carry out analytical verification at the serviceability and ultimate limit states to extend the validity of the proposed screw and specimen’s configurations.