Experimental Investigation and Reaction Kinetics Modeling of Biomass Formation, Substrate Consumption and Product Formation During Startup of Fixed-Bed Cultures with Immobilized Lactococcus lactis ssp. lactis

Faschian, Rebecca; Minden, Steven; Pörtner, Ralf

doi:10.4172/2155-9821.1000294

Cited by 2 publications

(9 citation statements)

References 35 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The dilution rates were chosen as 0.2, 0.4, 0.6, 0.8 and 0.9 times the maximum specific growth rate. For further details see [20].…”

Section: Bioreactor Set-up For Continuous Chemostat Cultivation Of Sumentioning

confidence: 99%

“…The principal set-up of the inner vessel is similar to the fixed-bed unit of the Multiferm (see above). For more details see [20]. Again, the borosilicate carrier (VitraPOR ® , ROBU ® Glasfilter-Geräte GmbH) with a mean diameter of 4 mm was used.…”

Section: Ml-fixed-bed Bioreactormentioning

confidence: 99%

“…(1)). This bioreactor system was established and precisely described previously [17,20]. Each unit was filled with borosilicate carriers (VitraPOR ® , ROBU ® Glasfilter-Geräte GmbH) with a mean diameter of 4 mm.…”

Section: Multiferm Fixed-bed Systemmentioning

confidence: 99%

“…For each experiment a fresh pre-culture was prepared from a cryostock as described previously [20]. This preculture was used as inoculum for experiments in the different reactors.…”

Section: Inoculum Preparationmentioning

confidence: 99%

“…A previously determined correlation between OD and total cell concentration (TCC) was used to convert OD values to biomass content [20]: …”

Section: Biomassmentioning

confidence: 99%

See 4 more Smart Citations

Evaluation of Fixed-Bed Cultures with Immobilized Lactococcus Lactis ssp. Lactis on Different Scales

Faschian¹,

Eren²,

Minden³

et al. 2017

TOBIOTJ

Self Cite

View full text Add to dashboard Cite

Fixed-bed processes, where cells are immobilized within macroporous carriers, are a promising alternative to processes with suspended cells. A scale-up concept is presented in order to evaluate the performance as part of process design of fixed-bed processes. Therefore, Lactococcus lactis cultivation in chemostat and batch mode was compared to fixed bed cultures on three different scales, the smallest being the downscaled Multiferm with 10 mL fixed bed units, the second a 100 mL fixed-bed reactor and the third a pilot scale reactor with 1 L fixed bed volume. As expected, the volume specific lactate productivity of all cultivations was dependent on dilution rate. In suspension chemostat culture a maximum of 2.3 g·L -1 ·h -1 was reached. Due to cell retention in the fixed-beds, productivity increased up to 8.29 g·L -1 ·h -1 at a dilution rate of D = 1.16 h -1 (corresponding to 2.4·µ max ) on pilot scale. For all fixed bed cultures a common spline was obtained indicating a good scale-up performance.

show abstract

“…The dilution rates were chosen as 0.2, 0.4, 0.6, 0.8 and 0.9 times the maximum specific growth rate. For further details see [20].…”

Section: Bioreactor Set-up For Continuous Chemostat Cultivation Of Sumentioning

confidence: 99%

Section: Ml-fixed-bed Bioreactormentioning

confidence: 99%

Section: Multiferm Fixed-bed Systemmentioning

confidence: 99%

“…For each experiment a fresh pre-culture was prepared from a cryostock as described previously [20]. This preculture was used as inoculum for experiments in the different reactors.…”

Section: Inoculum Preparationmentioning

confidence: 99%

“…A previously determined correlation between OD and total cell concentration (TCC) was used to convert OD values to biomass content [20]: …”

Section: Biomassmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Fixed-Bed Cultures with Immobilized Lactococcus Lactis ssp. Lactis on Different Scales

Faschian¹,

Eren²,

Minden³

et al. 2017

TOBIOTJ

Self Cite

View full text Add to dashboard Cite

show abstract

Design and Operation of Fixed-Bed Bioreactors for Immobilized Bacterial Culture

Pörtner¹,

Faschian²

2019

Growing and Handling of Bacterial Cultures

Self Cite

View full text Add to dashboard Cite

Fixed-bed processes operated in perfusion, where cells are immobilized within macroporous carriers, are a promising alternative to processes with suspended microbial or mammalian cells. Their potential has been demonstrated for many purposes. Nevertheless, the number of industrial fixed-bed processes is quite small. To some extent, this is due to the lack of process development tools for fixed-bed processes. To fill this gap, a strategy was developed for the design and evaluation of relevant process parameters of fixed-bed processes. A scale-up concept is presented in order to evaluate the performance as part of process design of fixed-bed processes. This comprises fixed-bed reactors on three different scales, the smallest being the downscaled Multiferm with 10 mL fixed-bed units, the second a 100 mL fixed-bed reactor, and the third a pilot-scale reactor with 1 L fixed-bed volume. The performance of this concept will be discussed for fixed-bed cultures of lactic acid bacteria. Furthermore, a reaction kinetic model for the design of fixed-bed reactors will be presented.

show abstract

Experimental Investigation and Reaction Kinetics Modeling of Biomass Formation, Substrate Consumption and Product Formation During Startup of Fixed-Bed Cultures with Immobilized Lactococcus lactis ssp. lactis

Cited by 2 publications

References 35 publications

Evaluation of Fixed-Bed Cultures with Immobilized Lactococcus Lactis ssp. Lactis on Different Scales

Evaluation of Fixed-Bed Cultures with Immobilized Lactococcus Lactis ssp. Lactis on Different Scales

Design and Operation of Fixed-Bed Bioreactors for Immobilized Bacterial Culture

Contact Info

Product

Resources

About