Experimental investigation of a microfluidic driving system for bi-directional manipulation

Lin, Yu-Cheng; Wu, Wei-Da; Jen, Chun-Ping; Wu, Guang-Guo; Chang, Chong-Ching

doi:10.1016/j.sna.2003.10.078

Cited by 5 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microfluidic cartridge consists of two parts: the reservoir plate and the sensor chip. The reservoir plate is made of the widely used poly(methyl methacrylate) (PMMA) [10][11][12][13][14][15][16] where reservoirs and delivery channels are shaped by micromilling and sealed by thermal bonding. External dimensions of the cartridge are 100 Â 40 Â 7 (in millimeters) and the reservoirs exhibit a volume capacity of around 100 mL.…”

Section: Microfluidic Cartridge Fabricationmentioning

confidence: 99%

Lab-on-a-chip for multiplexed biosensing of residual antibiotics in milk

et al. 2009

View full text Add to dashboard Cite

A multiplexed immunoassay-based antibiotic sensing device integrated in a lab-on-a-chip format is described. The approach is multidisciplinary and involves the convergent development of a multiantibiotic competitive immunoassay based on sensitive wavelength interrogated optical sensor (WIOS) technology and a polymer-based self-contained microfluidic cartridge. Immunoassay solutions are pressure-driven through external and concerted actuation of a single syringe pump and multiposition valve. Moreover, the use of a novel photosensitive material in a 'one step' fabrication process allowed the rapid fabrication of microfluidic components and interconnection port simultaneously. Pre-filled microfluidic cartridges were used as binary response rapid tests for the simultaneous detection of three antibiotic families -sulfonamides, fluoroquinolones and tetracyclines -in raw milk. For test interpretation, any signal lower than the threshold value obtained for the corresponding Maximum Residue Limit (MRL) concentration (100 mg L À1 ) was considered negative for a given antibiotic. The reliability of the multiplexed detection system was assessed by way of a validation test carried out on a series of six blind milk samples. A test accuracy of 95% was calculated from this experiment. The whole immunoassay procedure is fast (less than 10 minutes) and easy to handle (automated actuation).

show abstract

Section: Microfluidic Cartridge Fabricationmentioning

confidence: 99%

Lab-on-a-chip for multiplexed biosensing of residual antibiotics in milk

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Recently, micro total analysis system (m-TAS) and lab-on-achip (LOC) technologies have been applied to a broader scope for efficient molecular biological analyses and medical diagnoses. [1][2][3][4] In addition, active technologies, which are employed to actively move and concentrate the analyte at selected locations inside LOC and m-TAS by applying external forces through capillarity, 5 electric field, 6 hydrodynamics, 7 etc., have been also developed for enhancing molecular interactions, hybridization and separation.…”

Section: Introductionmentioning

confidence: 99%

Electric field isolator (EFI) for isolated and electrophoretic manipulation of charged biomolecules

et al. 2007

View full text Add to dashboard Cite

This paper describes a novel technology-an electric field isolator (EFI)-that can be used for achieving isolated and electrophoretic manipulation of charged biomolecules inside a selected microscopic location. The EFI is a ground ring-shaped electrode (RE) surrounding a centre electrode (CE), which is comprised of a functional unit. When the CE is powered, the ground RE can inhibit the electric field from spreading to the neighbouring functional units. Therefore, the electrophoretic movement of the charged molecules in an electric field, which is based on the principle similar to that of electrophoresis, can be isolated inside a selected location. The ground RE causing this phenomenon is referred to as the EFI. In this paper, we clearly show the functionality of the EFI with mathematical and experimental studies.

show abstract