Impinging stream technique has been widely used in engineering industries. Insufficient data are available on the effects of dynamic inflow conditions on the flow and turbulence characteristics of an impinging stream reactor. In this study, we investigate and discuss the flow and turbulence characteristics of an impinging stream reactor with dynamic inlet velocity variation, e.g., sinusoidal, parabolic, step or triangular variation. The effects of period, amplitude, phase difference, mean inlet velocity and type of dynamic inlet velocity variation on the motional behaviors of the impinging surface and the mean turbulence kinetic energy (k) of the impingement region are investigated and discussed using particle image velocimetry (PIV) and computational fluid dynamics (CFD) at various values of L/D (the ratio of impinging spacing to nozzle diameter). The results show that the impinging surface makes back-and-forth motions in impinging stream reactors with dynamic inlet velocity variation. The mean k of the impingement region during one period is dominated by both the inlet velocity conditions and the geometric configuration. Dynamic inflow conditions bring more turbulence energy and pulsating characteristics to impinging zones over constant inlet velocity for an instantaneously moving impinging surface. Impinging stream reactors with dynamic inlet velocity variation provides more intense turbulence properties over conventional impinging stream reactors at the same mean inlet velocity. This work shows that the impinging streams with dynamic inlet velocity variation has strong potential for future relevant reactors and processes for engineering applications.