The usage of fossil fuels are rising rapidly and diminishing its reserves at the same phase. Moreover petroleum based fuels are emitting enormous toxic exhaust emissions which are perilous to the environment. This environmental pollution is causing triple impact on health of humans, plants and other living habitats. In the recent time, the plant based biodiesel are becoming a hopeful alternative renewable fuel especially with non-edible oils as feed stock, to replace the conventional diesel fuel. The present research paper aims to perform experimental evaluation of the exhaust emissions from direct injection diesel engine when madhuca indica oil methyl ester and its diesel blends used as fuel. Madhuca indica oil methyl ester (MIOME) was prepared through transesterification process using methyl alcohol and an experimental study was carried-out using a 4-stroke, single cylinder, compression ignition direct injection engine fuelled with MIOME, different blends (B20M, B40M, B60M and B100M) of MIOME and diesel fuel. The experimental tests revealed that the emission of B100M biodiesel has lower carbon monoxide (CO), particle pollution, and smoke opacity emissions when compared to diesel fuel. Biodiesels are naturally oxidized fuels and so emitting lower exhaust emissions due to its higher cetane number than diesel fuel with less or no sulphur content.