Crude oil, which is brought to the surface from underground with high cost and loss of time, contains water, gas and some solids. The most widely used method for separating the crude oil brought to the earth from water is the separation method, which is one of the gravitational separation methods, using corrugated plates.In this study, 3 different Re numbers (3033.058, 4766.2345, 6499.411) of 1st Processed crude oil sample, the ratio of 3 different hole diameters of the corrugated plates to the plate width (0.018,0.021,0.024) and 3 different mounting angles (0, 25, 50 degrees) parameters were taken into consideration and the effect of the relevant parameters on the separation efficiency was investigated. In order to reduce the number of experiments and save on cost and experiment time, without affecting the probability of finding the highest value of the separation efficiency, the Response Surface Method (RSM) was optimized with the Box-Behnken method and relevant trial designs were created.In line with these experimental designs, the relevant experiments were carried out in duplicate.