Experimental Investigation of Pulsatility Effect on the Deformability and Hemolysis of Blood Cells

Kang, Yang Jun; Kim, Myoung Gon; Son, Kuk Hui; Lim, Choon Hak; Son, Ho Sung; Yoon, Sang Youl; Kwon, Hyuk Sang; Yang, Sung

doi:10.1111/j.1525-1594.2009.00974.x

Cited by 17 publications

(14 citation statements)

References 24 publications

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“…Cell damage observed even today with prosthetic heart valves, 15,39 cardiopulmonary bypass, 25 and artificial kidneys contributes to anemia, along with the toxic effects associated with free hemoglobin in the circulatory system. 38 The cause of mechanical trauma to blood cells has been attributed to large frictional forces or shear stresses, 30 which are associated with the flow of blood through such devices.…”

Section: Introductionmentioning

confidence: 99%

Significance of Extensional Stresses to Red Blood Cell Lysis in a Shearing Flow

2011

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Traditionally, an empirical power-law model relating hemolysis to shear stress and exposure time has been used to estimate hemolysis related to flow--however, this basis alone has been insufficient in attempts to predict hemolysis through computational fluid dynamics. Because of this deficiency, we sought to re-examine flow features related to hemolysis in a shearing flow by computationally modeling a set of classic experiments performed in a capillary tube. Simulating 21 different flows of varying entrance contraction ratio, flowrate and viscosity, we identified hemolysis threshold streamlines and analyzed the stresses present. Constant damage thresholds for radial and axial extensional stresses of approximately 3000 Pa for exposure times on the order of microseconds were observed, while no such threshold was found for the maximum shear stress or gradient of the shear stress. The extensional flow seen at the entrance of the capillary appears to be most consistently related to hemolysis. An account of how extensional stresses can lead to lysis of a red cell undergoing tank-tread motion in a shearing flow is provided. This work shows that extensional components of the stress tensor are integral in causing hemolysis for some flows, and should be considered when attempting to predict hemolysis computationally.

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Section: Introductionmentioning

confidence: 99%

Significance of Extensional Stresses to Red Blood Cell Lysis in a Shearing Flow

2011

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“…According to previous studies on blood biophysical properties including viscosity, [1][2][3][4][5][6] viscoelasticity, [7][8][9][10][11][12] deformability, [13][14][15][16][17][18] aggregation, [19][20][21][22] and erythrocyte sedimentation rate (ESR), [23][24][25][26][27] various cardiovascular diseases cause changes in the biophysical properties of the blood. The biophysical properties of the blood have been measured to effectively monitor disease states or their progresses.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic-based measurement of erythrocyte sedimentation rate for biophysical assessment of blood in an in vivo malaria-infected mouse

Kang

Lee

2014

Biomicrofluidics

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This study suggests a new erythrocyte sedimentation rate (ESR) measurement method for the biophysical assessment of blood by using a microfluidic device. For an effective ESR measurement, a disposable syringe filled with blood is turned upside down and aligned at 180 with respect to gravitational direction. When the blood sample is delivered into the microfluidic device from the top position of the syringe, the hematocrit of blood flowing in the microfluidic channel decreases because the red blood cell-depleted region is increased from the top region of the syringe. The variation of hematocrit is evaluated by consecutively capturing images and conducting digital image processing technique for 10 min. The dynamic variation of ESR is quantitatively evaluated using two representative parameters, namely, time constant (k) and ESR-area (A ESR ). To check the performance of the proposed method, blood samples with various ESR values are prepared by adding different concentrations of dextran solution. k and A ESR are quantitatively evaluated by using the proposed method and a conventional method, respectively. The proposed method can be used to measure ESR with superior reliability, compared with the conventional method. The proposed method can also be used to quantify ESR of blood collected from malaria-infected mouse under in vivo condition. To indirectly compare with the results obtained by the proposed method, the viscosity and velocity of the blood are measured using the microfluidic device. As a result, the biophysical properties, including ESR and viscosity of blood, are significantly influenced by the parasitemia level. These experimental demonstrations support the notion that the proposed method is capable of effectively monitoring the biophysical properties of blood. V C 2014 AIP Publishing LLC. [http://dx

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“…This is in accordance with the finding by Kang et al, who showed that hemolysis was increased with prolonged CPB duration irrespective of flow condition. 26 …”

Section: Interpretation Of Heterogenous Resultsmentioning

confidence: 97%