Both experimental and simulated studies of the micro-discharge (MD) are carried out in a DBD with a pin-to-pin gap of 3.5 mm, ignited by a sinusoidal voltage with a peak voltage of 10 kV and a driving frequency of 5 kHz. A statistical result has shown that the probability of the single current pulse in the positive half-period (HP) reaches 73.6% under these conditions. Experimental results show that the great intensity of the luminous is concentrated on the dielectric surface and the tip of the metal electrode. A one-dimensional (1D) plasma fluid model is implemented by coupling the species continuity equations, electron energy density equations, Poisson equation, and Helmholtz equations to analyze the MD dynamics on the micro-scale. The simulated results are in good qualitative agreement with the experimental results. The simulated results show that the MD dynamics can be divided into three phases: the Townsend phase, the streamer propagation phase, and the discharge decay phase. During the streamer propagation phase, the electric field and electron density increase with the streamer propagation from the anode to the cathode, and their maximal values reach 625.48 Td and 2.31×1019 m−3 as well as 790.13 Td and 3.58×1019 m−3 in the positive and negative HP, respectively. Furthermore, a transient glow-like discharge is detected around the anode during the same period of the streamer propagation. The formation of transient glow-like discharge is attributed to electrons drifting back to the anode, which is driven by the residual voltage between the air gap.