Surface roughness elements on hypersonic vehicles can cause early boundary layer transition, increasing wall skin friction and heat flux and affecting aircraft range and thermal protection systems. Accurate prediction of the transition caused by these roughness elements is crucial for the design of hypersonic vehicles. In this work, wind tunnel experiments on isolated roughness-induced boundary layer transition at Ma = 6 are conducted. Infrared thermography and high-frequency pressure sensors are utilized to investigate the effects of different roughness element configurations (cylindrical, diamond, ramp) on the hypersonic boundary layer instability and transition. The experimental results show that all three roughness elements can effectively enhance the generation of second mode waves and promote boundary layer transition. Compared to smooth surfaces, they exhibit similar frequency band range, faster growth, and earlier saturation. Among them, the ramp roughness element most effectively triggers the boundary layer transition, with a relatively small heat flux increase. Furthermore, bispectral analysis illustrates that all three roughness elements undergo self-interactions that lead to spectral broadening, ultimately resulting in boundary layer transitions.