Experimental Investigation on the Mechanical Behavior of Bovine Bone Using Digital Image Correlation Technique

Chen, Yuxi; Yang, Diansen; Ma, Yongshang; Tan, Xianjun; Shi, Zhan; Li, Taoran; Si, Huaijun

doi:10.1155/2015/609132

Cited by 9 publications

(8 citation statements)

References 10 publications

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“…Combination treatment of dimethyloxalglycine and butyrate improved bone regeneration and angiogenesis concomitant with increasing expression levels of VEGF in a rat model 41 whilst Chen Y. et al . 42 showed that the presence of butyrate increased angiogenesis in diabetic rat hearts, also inhibiting cardiac hypertrophy. It would appear the micro-environmental concentration of sodium butyrate within the tissue might determine its biological activity as we also showed that concentrations above 10 μg/ml were not able any more to stimulate angiogenesis in vitro .…”

Section: Discussionmentioning

confidence: 99%

Low Concentration of Sodium Butyrate from Ultrabraid+NaBu suture, Promotes Angiogenesis and Tissue Remodelling in Tendon-bones Injury

Liu

Andrade

Castro

et al. 2016

Sci Rep

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Sodium butyrate (NaBu), a form of short-chain fatty acid (SCFA), acts classically as a potent anti-angiogenic agent in tumour angiogenesis models, some authors demonstrated that low concentrations of NaBu may contribute to healing of tendon-bone injury in part at least through promotion of tissue remodelling. Here, we investigated the effects of low-range concentrations of NaBu using in vitro and in vivo assays using angiogenesis as the primary outcome measure and the mechanisms through which it acts. We demonstrated that NaBu, alone or perfused from the UltraBraid+NaBu suture was pro-angiogenic at very low-range doses promoting migration, tube formation and cell invasion in bovine aortic endothelial cells (BAECs). Furthermore, cell exposure to low NaBu concentrations increased expression of proteins involved in angiogenic cell signalling, including p-PKCβ1, p-FAK, p-ERK1/2, p-NFκβ, p-PLCγ1 and p-VEGFR2. In addition, inhibitors of both VEGFR2 and PKCβ1 blocked the angiogenic response. In in vivo assays, low concentrations of NaBu induced neovascularization in sponge implants in mice, evidenced by increased numbers of vessels and haemoglobin content in these implants. The findings in this study indicate that low concentrations of NaBu could be an important compound to stimulate angiogenesis at a site where vasculature is deficient and healing is compromised.

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Section: Discussionmentioning

confidence: 99%

Low Concentration of Sodium Butyrate from Ultrabraid+NaBu suture, Promotes Angiogenesis and Tissue Remodelling in Tendon-bones Injury

Liu

Andrade

Castro

et al. 2016

Sci Rep

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“…() in 1999. DVC is a 3D‐extension of the 2D digital image correlation (DIC) technique, a widely employed method for measurement of full‐field displacement and strain fields in a variety of materials subjected to mechanical or thermal loads (Hild & Roux, ; Pan et al ., ; Chen et al ., ; Palanca et al ., ; Hensley et al ., ). For a given material sample under applied load, DIC provides a surface measurement of deformation or strain, whereas DVC is able to provide full‐field displacement and strain fields throughout the interior of the sample in 3D (Bay et al ., ; Bay, ).…”

Section: Biomechanical Xct Analysis Of Bone Regenerationmentioning

confidence: 97%

“…Although time-lapsed mechanical testing and XCT imaging revealed important 3D visual information on the deformation of bone and biomaterial structures, a quantification of the full-field displacement and strain was only achieved with the development of digital volume correlation (DVC) by Bay et al (1999) in 1999. DVC is a 3D-extension of the 2D digital image correlation (DIC) technique, a widely employed method for measurement of full-field displacement and strain fields in a variety of materials subjected to mechanical or thermal loads (Hild & Roux, 2006;Pan et al, 2009;Chen et al, 2015;Palanca et al, 2015;Hensley et al, 2017). For a given material sample under applied load, DIC provides a surface measurement of deformation or strain, whereas DVC is able to provide full-field displacement and strain fields throughout the interior of the sample in 3D (Bay et al, 1999;Bay, 2008).…”

Section: Digital Volume Correlationmentioning

confidence: 99%

Applications of X‐ray computed tomography for the evaluation of biomaterial‐mediated bone regeneration in critical‐sized defects

Fernández

Witte²,

Tozzi

2019

Journal of Microscopy

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Bone as such displays an intrinsic regenerative potential following fracture; however, this capacity is limited with large bone defects that cannot heal spontaneously. The management of critical‐sized bone defects remains a major clinical and socioeconomic need with osteoregenerative biomaterials constantly under development aiming at promoting and enhancing bone healing. X‐ray computed tomography (XCT) has become a standard and essential tool for quantifying structure–function relationships in bone and biomaterials, facilitating the development of novel bone tissue engineering strategies. This paper presents recent advancements in XCT analysis of biomaterial‐mediated bone regeneration. As a noninvasive and nondestructive technique, XCT allows for qualitative and quantitative evaluation of three‐dimensional (3D) scaffolds and biomaterial microarchitecture, bone growth into the scaffold as well as the 3D characterisation of biomaterial degradation and bone regeneration in vitro and in vivo. Furthermore, in combination with in situ mechanical testing and digital volume correlation (DVC), XCT demonstrated its potential to better understand the bone–biomaterial interactions and local mechanics of bone regeneration during the healing process in relation to the regeneration achieved in vivo, which will likely provide valuable knowledge for the development and optimisation of novel osteoregenerative biomaterials. Lay Description Bone, being a dynamically adaptable material, displays excellent regenerative properties following fracture. However, the self‐healing capacity of bone becomes more difficult with large bone defects. Those defects are common and occur in many clinical situations; hence, biomaterials are mostly used to restore both bone structure and function in the defect site. X‐ray computed tomography (XCT) is a powerful tool to evaluate bone regeneration in critical‐sized defects after the implantation of biomaterials, allowing to an improved understanding of the regeneration process following different bone tissue engineering approaches. This paper focuses on recent advancements in XCT analysis to characterise biomaterial‐mediated bone regeneration in critical‐sized defects. XCT supports three‐dimensional (3D) analysis of biomaterials, scaffolds and regenerated bone microarchitecture, as well as bone ingrowth into the scaffold. As a nondestructive technique, XCT allows for a 3D characterisation of biomaterial degradation and bone regeneration over time. In addition, XCT combined with in situ mechanical experiments and digital volume correlation (DVC) provides a 3D evaluation and quantification of bone–biomaterial interactions and deformation mechanisms during the regeneration process. This remains essential for the development and enhancement of novel biomaterials able to produce bone that is comparable with the native tissue they aim to replace.

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“…Bovine bones, especially bovines shown in Fig. 1, are often used as experimental samples to study the mechanical properties of bones, which is generally viewed as an acceptable surrogate for human bone (Chen et al, 2015). Bovine bones are selected mainly because: (a) The researcher has relatively little further ethical requirement to meet; (b) the close relationship between the mechanical properties of bovine bone and human bone; (c) large size makes it easy to test the whole bone in large-size.…”

Section: Test Samplementioning

confidence: 99%

“…Bone is a multi-phase, lamellar and discontinuous composite structure (Sun et al, 2018). There are many aspects to study the mechanical behaviour of bones (Szabó and Thurner, 2013;Chen et al, 2015), including:…”

Section: Introductionmentioning

confidence: 99%

Experimental Investigation on the Failure Mechanism of Bovine Bone by Three-point Bending Tests

Li¹,

Gao²,

Li³

et al. 2020

American Journal of Biochemistry and Biotechnology

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Bone damage is a common type of sport injury. The loading rate affects the type of fracture and the degree of soft tissue injury. In view of the problem of whole bone bending damage and failure, this paper introduces the test method of bovine bone bending and the mechanism of bending failure under different loading rates is analyzed through experimental research and theoretical analysis. The results of three-point bending failure test of bovine bone are given and the influence of loading rate on bending failure is discussed. The results show that there are three main deformation and failure modes of bovine bone under different loading rates, namely the mode of fracture failure under high loading rate, the mode of shear failure under medium loading rate and the mode of local wedge failure under low loading rate. With the increase of loading rate, the greater the failure force is applied on the bone, the smaller the destructive deflection of the bone will be. The maximum strain of bone decreases with the increase of loading rate, while the flexural modulus of bone increases gradually, which indicates that the loading rate has an effect on the brittleness of the whole bone. For the bending model of the whole bovine bone, the prediction result of elliptical cross-section method is more in accordance with the actual situation than that of circular cross-section method.

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Experimental Investigation on the Mechanical Behavior of Bovine Bone Using Digital Image Correlation Technique

Cited by 9 publications

References 10 publications

Low Concentration of Sodium Butyrate from Ultrabraid+NaBu suture, Promotes Angiogenesis and Tissue Remodelling in Tendon-bones Injury

Low Concentration of Sodium Butyrate from Ultrabraid+NaBu suture, Promotes Angiogenesis and Tissue Remodelling in Tendon-bones Injury

Applications of X‐ray computed tomography for the evaluation of biomaterial‐mediated bone regeneration in critical‐sized defects

Experimental Investigation on the Failure Mechanism of Bovine Bone by Three-point Bending Tests

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