Experimental leprosy in monkeys. II. Longitudinal serological observations in sooty mangabey monkeys

Abstract

In this study, 11 SMM were grouped and inoculated with differing doses of SMM-origin My cobacterium /eprae (ML) between 4•5 x 10 8 and I x 10 9 by either combined IV IIC routes or by IV or IC route alone. The combined route was the most effective in eliciting progressive, disseminated LL leprosy. In all, 6 of 7 SMM inoculated by the combined routes developed leprosy requiring treatment at some point. Only 1 of 4 inoculated by a single route developed persisting leprosy requiring chemotherapy. Either no disease or spontaneous regression of initial disease occurred in the other 3 animals inoculated by a single route. Doses in excess of I x 10 9 ML were more effective than lesser doses. An association was observed between the development of IgG anti-PGL-I ELISA OD values and resistance to leprosy and between IgM anti-PGL-I and leprosy progression or susceptibility. Serum PGL-I antigen levels, determined by dot ELISA, paralleled disease severity longitudinally. High positive OD values of anti-LAM IgG prior to ML inoculation were observed in the majority ofleprosy susceptible SMM in contrast to negative levels in more resistant animals. Anti LAM IgG OD values exceeded the positive cutoff point after inoculation in 5 of II SMM; 3 of these 5 had concurrent detectable serum levels of PGL-I antigen. We previously observed that longitudinal serologic data from sooty mangabey monkeys (SMM) (Cercocebus torq uatus aty s) experimentally inoculated with My cobacterium /eprae (ML) may provide indicators for susceptibility to leprosy. l , 2 In that study,

Classic AIDS in a Sooty Mangabey after an 18-Year Natural Infection

“…They were used for experimental leprosy infection (17,18) because of their unique susceptibility to Mycobacterium leprae (16,25,46). A high prevalence of SIV infection has been reported for the YNPRC colony (10), and 90% of SMs were SIVsm infected when they were moved to TNPRC (29).…”

SIV progenitor evolution toward HIV: A humanized mouse surrogate model for SIVsm adaptation toward HIV‐2

“…The SIVsmE041 (GenBank accession ) used in this study was obtained from a 21‐year‐old sooty mangabey euthanized for displaying clinical signs of AIDS and then propagated in sooty mangabey PBMC creating a true primary isolate comprised of a population of closely related viruses.…”

Modeling the evolution of SIV sooty mangabey progenitor virus towards HIV-2 using humanized mice

“…SM E041 was a male sooty mangabey born in January 1981 at the Yerkes Primate Research Center (YNPRC) for experimental leprosy infection and transferred in 1983 to the Tulane National Primate Research Center (TNPRC) (Gormus et al, 1995a; 1995b). E041 was infected with SIVsm by natural spread between group-housed mangabeys before leprosy experiments began at YNPRC and neither clinical nor histological evidence of leprosy was seen during 18 years of observation (Fultz et al, 1986; Ling et al, 2004; Murphey-Corb et al, 1986).…”