Experimental model for reproduction of canine visceral leishmaniosis by Leishmania infantum

Fernández-Cotrina, Javier; Iniesta, Virginia; Belinchón-Lorenzo, Silvia; Muñoz-Madrid, Rubén; Serrano, Francisco; Parejo, Juan Carlos; Gómez-Gordo, Luis; Soto, Manuel; Alonso, Carlos; Gómez-Nieto, Luis Carlos

doi:10.1016/j.vetpar.2012.10.002

Cited by 32 publications

(36 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Five dogs presented exfoliative dermatitis (D240: n = 2; D270: n = 5) associated with diffuse to general non inflammatory alopecia (D120: n = 3), which is the most prevalent cutaneous manifestation of CanL (Koutinas et al, 1999;Rodríguez-Cortés et al, 2007;Fernández-Cotrina et al, 2013). After D300 and D360, the main systemic signs of CanL were observed, such as asthenia (n = 2), splenomegaly (n = 8), conjunctivitis (n = 5), generalized muscular atrophy (n = 3), temporal muscles atrophy (n = 2), onychogryphosis (n = 5), alopecia (n = 6) and plantar hyperkeratosis (n = 5), reflecting the wide spectrum of clinical manifestations produced during natural infection (Hommel et al, 1995).…”

Section: Resultsmentioning

confidence: 99%

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

Hernández

Montoya

Checa

et al. 2015

Veterinary Parasitology

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

Hernández

Montoya

Checa

et al. 2015

Veterinary Parasitology

View full text Add to dashboard Cite

“…In addition, the administration of the same challenge dose from the same batch of parasites to each dog on the same day by the same route reduced challenge variability, and allowed the effects of the vaccine to be studied in highly standardized conditions [38]. …”

Section: Discussionmentioning

confidence: 99%

The protective immune response produced in dogs after primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) remains effective against an experimental challenge one year later

Martin

Vouldoukis

Moreno

et al. 2014

Vet Res

View full text Add to dashboard Cite

Control of canine leishmaniasis is an important objective for the benefit of dogs living in or visiting endemic areas and for public health because of the zoonotic nature of this disease. Resistance or susceptibility to developing canine leishmaniasis after exposure to Leishmania infantum is primarily determined by the ability of the immune system to develop an appropriate Th1-dominated specific response to the parasite. For this reason there is a need for effective canine vaccines that can decrease the number of dogs developing progressive infections. In this study, we followed the impact of the LiESP/QA-21 canine vaccine (composed of excreted-secreted proteins of L. infantum and the QA-21 saponin adjuvant), recently launched commercially in Europe, on selected humoral and cellular immune parameters following an infectious intravenous challenge with L. infantum promastigotes administered one year after the primary vaccine course. We also followed parasitological parameters to determine the parasitological status of the challenged dogs. In contrast to controls, vaccinated dogs retained significantly stronger cell-mediated immune responses against the parasite despite a virulent challenge and had significantly lower mean parasite burdens at the end of the study, associated with a lower probability of developing active infections. These results confirm that the immune responses generated by vaccination with LiESP/QA-21 are still effective against an intravenous challenge one year after the primary vaccine course.

show abstract

“…Two isolates named IPER/ES/2012/BOS1FL1 (BOS1FL1) and IPER/ES/2012/POL2FL7 (POL2FL7) were isolated from P. perniciosus captured in the focus of Madrid using CDC light traps. BCN150 is a well characterized strain (M/CAN/ES/96/BCN150 zymodeme MON-1) that was isolated from a dog with active VL [21]. This strain has traditionally been used in our laboratory’s experiments related to VL [22-24] and by others in canine leishmaniosis studies [21,25,26].…”

Section: Methodsmentioning

confidence: 99%

Characterisation of the ex vivo virulence of Leishmania infantum isolates from Phlebotomus perniciosus from an outbreak of human leishmaniosis in Madrid, Spain

et al. 2014

View full text Add to dashboard Cite

BackgroundSince mid 2009, an outbreak of human leishmaniosis in Madrid, Spain, has involved more than 560 clinical cases. Many of the cases occurred in people who live in areas around a newly constructed green park (BosqueSur). This periurban park provides a suitable habitat for sand flies (the vectors of Leishmania infantum). Indeed, studies of blood meals from sand flies captured in the area showed a strong association between the insect vector, hares or rabbits, and humans in the area. Interestingly, up to 70% of cases have been found in immunocompetent patients (aged between 46-60 years). This study was designed to evaluate the ex vivo virulence of the L. infantum isolates from Phlebotomus perniciosus captured in this area of Madrid.MethodsMurine macrophages and dendritic cells were infected ex vivo with L. infantum strain BCN150, isolate BOS1FL1, or isolate POL2FL7. At different times after infection, the infection indices, cytokine production (IL-12p40 and IL-10), NO release and arginase activities were evaluated.ResultsUsing an ex vivo model of infection in murine bone marrow-derived cells, we found that infection with isolates BOS1FL1 and POL2FL7 undermined host immune defence mechanisms in multiple ways. The main factors identified were changes in both the balance of iNOS versus arginase activities and the equilibrium between the production of IL-12 and IL-10. Infection with isolates BOS1FL1 and POL2FL7 also resulted in higher infection rates compared to the BCN150 strain. Infection index values at 24 h were as follows: BCN150-infected cells, 110 for infected MØ and 115 for infected DC; BOS1FL1-infected cells, 300 for infected MØ and 247 for infected DC; and POL2FL7-infected cells, 275 for infected MØ and 292 for infected DC.ConclusionsOur data indicate that L. infantum isolates captured from this endemic area exhibited high virulence in terms of infection index, cytokine production and enzymatic activities involved in the pathogenesis of visceral leishmaniosis. Altogether, these data provide a starting point for the study of the virulence behaviour of parasites (BOS1FL1 and POL2FL7) isolated from P. perniciosus during the outbreak of human leishmaniosis in Madrid, Spain, and their involvement in infecting immunocompetent hosts.

show abstract

Experimental model for reproduction of canine visceral leishmaniosis by Leishmania infantum

Cited by 32 publications

References 40 publications

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques

The protective immune response produced in dogs after primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) remains effective against an experimental challenge one year later

Characterisation of the ex vivo virulence of Leishmania infantum isolates from Phlebotomus perniciosus from an outbreak of human leishmaniosis in Madrid, Spain

Contact Info

Product

Resources

About