Experimental model of heterotopic ossification in Wistar rats

Zotz, Talita Gianello Gnoato; Paula, Josué Bruginski de; Moser, Auristela Duarte de Lima

doi:10.1590/s0100-879x2012007500049

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…These are a lot of kinds of models of AHO that have been reported. (Zotz et al, 2012;Kan & Kessler, 2011) Achilles tenotomy model is widely used. McClure applied the model to mice and found that AHO developed by 10th weeks in 1983.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Nf-ҝb prevents trauma-induced heterotopic ossification in rat model

Ju¹,

Xue

et al. 2017

Preprint

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Background. To find a better prophylactic regimen, the pathogenesis of acquired heterotopic ossification (AHO) must be more understood. To date, AHO formation is largely thought to be related to inflammation, which is activated by trauma, resulting in AHO by up-regulation of pro-osteogenic genes. Methods. Brain-traumatic/burn/tenotomy model is firstly used in experiment. At first, 44 rats were randomly divided into two groups: E group and C group. Two rats in every group were euthanized during second, third, fourth, sixth, eighth, tenth weeks for collecting tendon. The remaining rats survived until tenth week for X-Ray radiation examination to confirm the size of AHO.Then, 124 rats were randomly divided into four group: P group, L group, M group, H group. The three rats of every group were euthanized during every week of the first seven weeks for collecting tendon to detect P65 protein. The remaining rats survived until tenth week for X-Ray examination to confirm the size of AHO. Results. The success rate of Brain-traumatic/Burn/Tenotomy model is 100%. Difference of P65 expression in E group and in C group are statistically significant,and that in E group is higher.Pharmacologic inhibition of Nf-ҝb signaling pathway limits AHO formation, and that The bone formation content of M group is decreased. Conclusion. Brain-traumatic/Burn/Tenotomy model is highly reliable.Results indicate that the Nf-ҝb /p65 signaling response occurs in the forming process of AHO. PDTC limits formation of AHO. The most effective concentration is 6mg/ml for local injection.

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Section: Discussionmentioning

confidence: 99%

Inhibition of Nf-ҝb prevents trauma-induced heterotopic ossification in rat model

Ju¹,

Xue

et al. 2017

Preprint

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“…The first blood collection was from intracardiac puncture, 1 week before HO induction ( 16 ), giving the animals time to recover from the blood collection and any eventual blood loss. The experiment lasted 43 days: day 1 was the first blood collection, day 8 was HO induction, and day 43 was the day the animals were killed.…”

Section: Methodsmentioning

confidence: 99%

“…For the implant, a thin needle (0.3×16 mm) for insulin injection was inserted perpendicularly to the ventral side of the thigh. All animal groups received 0.35 mL of BM and were given oral doses of 20 mg·kg −1 ·24 h −1 dipyrone (500 mg/mL, Eurofarma, Brazil) during the first 3 days for pain relief after the BM collection procedure ( 16 ).…”

Section: Methodsmentioning

confidence: 99%

Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats

Zotz

Paula

2015

Braz J Med Biol Res

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Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues, resulting in joint mobility deficit and pain. Different treatment modalities have been tried to prevent HO development, but there is no consensus on a therapeutic approach. Since electrical stimulation is a widely used resource in physiotherapy practice to stimulate joint mobility, with analgesic and anti-inflammatory effects, its usefulness for HO treatment was investigated. We aimed to identify the influence of electrical stimulation on induced HO in Wistar rats. Thirty-six male rats (350-390 g) were used, and all animals were anesthetized for blood sampling before HO induction, to quantify the serum alkaline phosphatase. HO induction was performed by bone marrow implantation in both quadriceps of the animals, which were then divided into 3 groups: control (CG), transcutaneous electrical nerve stimulation (TENS) group (TG), and functional electrical stimulation (FES) group (FG) with 12 rats each. All animals were anesthetized and electrically stimulated twice per week, for 35 days from induction day. After this period, another blood sample was collected and quadriceps muscles were bilaterally removed for histological and calcium analysis and the rats were killed. Calcium levels in muscles showed significantly lower results when comparing TG and FG (P<0.001) and between TG and CG (P<0.001). Qualitative histological analyses confirmed 100% HO in FG and CG, while in TG the HO was detected in 54.5% of the animals. The effects of the muscle contractions caused by FES increased HO, while anti-inflammatory effects of TENS reduced HO.

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“…HO formation in rodents has been achieved but included cytokine modulation of the animal 35. The model produced by Tannous et al used local blast to the lower limb and therefore systemic causes of blast related HO were not taken into account 36.…”

Section: Required Researchmentioning

confidence: 99%

Heterotopic ossification: a systematic review

Edwards

Clasper²

2014

J R Army Med Corps

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Heterotopic ossification (HO) is the formation of mature lamellar bone in extraskeletal soft tissues. It was first described 1000 years ago in the healing of fractures, and in relation to military wounds, texts from the American Civil War and World War I refer to HO specifically. It continues to cause problems to injured service personnel; the consequences of wound and soft tissue complications in traumatic amputations pose particular problems to rehabilitation and prosthetic use. While HO is seen in rare genetic conditions, it is most prevalent after joint replacement surgery and trauma. In the civilian setting HO has been commonly described in patients after traumatic brain injuries, spinal cord injuries and burns. Militarily, as a consequence of recent operations, and the characteristic injury of blast-related amputations, a renewed interest in HO has emerged due to an increased incidence seen in casualties. The heterogeneous nature of a blast related amputation makes it difficult for a single aetiological event to be identified, although it is now accepted that blast, amputation through the zone of injury, increased injury severity and associated brain injuries are significant risk factors in HO formation. The exact cellular event leading to HO has yet to be identified, and as a consequence its prevention is restricted to the use of anti-inflammatory medication and radiation, which is often contraindicated in the acute complex military casualty. A systematic review in PubMed and the Cochrane Database identified research articles related to HO to illustrate the military problem of HO and its management, current research concepts and experimental theories regarding HO. This also served as a gap analysis providing the researchers detail of any knowledge deficit in this field, in particular to the military aspects of HO; 637 out of 7891 articles initially identified that referenced HO were relevant to this review.

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Experimental model of heterotopic ossification in Wistar rats

Cited by 8 publications

References 27 publications

Inhibition of Nf-ҝb prevents trauma-induced heterotopic ossification in rat model

Inhibition of Nf-ҝb prevents trauma-induced heterotopic ossification in rat model

Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats

Heterotopic ossification: a systematic review

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